Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,100	C→T	V768V (GTG→GTA)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,100	0	C	T	100.0%	76.1 / NA	22	V768V (GTG→GTA)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/13);  total (9/13)

GCACGAACATACCAGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGT  >  NC_000913/482026‑482170                                                                           |                                                                       gcaCGAACATACCAGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGa                                                                  >  2:107417/1‑81 (MQ=255)   aCGAACATACCAGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGAcc                                                                <  2:107378/81‑1 (MQ=255)      aaCATACCAGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACcgcg                                                             <  2:107379/81‑1 (MQ=255)         aTACCAGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTc                                                          <  1:107380/81‑1 (MQ=255)            ccAGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGAt                                                       <  2:107381/81‑1 (MQ=255)              aGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATa                                                      >  1:107429/1‑80 (MQ=255)                           atcCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGCTAAAGTCGTTCACAt                                         <  1:107382/80‑1 (MQ=255)                              cGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCt                                     <  2:107383/81‑1 (MQ=255)                                gcagcaTACGGTATTTCGCTTCTGCCATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTgc                                   >  1:107435/1‑81 (MQ=255)                                  agcaTACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTgccg                                 >  2:107425/1‑81 (MQ=255)                                   gcaTACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTgccgc                                <  1:107384/81‑1 (MQ=255)                                    caTACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTgccgcc                               <  1:107385/81‑1 (MQ=255)                                     aTACGGTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGccc                              >  1:107426/1‑81 (MQ=255)                                          gTATTTCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGc                         <  2:107386/81‑1 (MQ=255)                                             tttCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAgc                      <  1:107387/81‑1 (MQ=255)                                               tCGCTTCTGACATGACATAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAgcgc                    >  1:107431/1‑81 (MQ=255)                                                        acatgacatAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAgtg            <  2:107388/80‑1 (MQ=255)                                                          atgacatAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAgtggtg         <  2:107389/81‑1 (MQ=255)                                                             acatAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGtt       >  1:567487/1‑80 (MQ=255)                                                               atAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAAt    <  2:107390/81‑1 (MQ=255)                                                               atAAACTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAAt    >  2:107442/1‑81 (MQ=255)                                                                 aaaCTTTCTTTACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGt  >  2:107418/1‑81 (MQ=255)                                                                           |                                                                       GCACGAACATACCAGTCGCCGATATCATCCGGCAGCATACGGTATTTCGCTTCTGACATGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGT  >  NC_000913/482026‑482170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑