Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,153	C→T	S33S (AGC→AGT)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,153	0	C	T	97.2%	123.4 / NA	36	S33S (AGC→AGT)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (25/10);  minor base A (0/1);  total (25/11)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCC  >  NC_000913/498076‑498230                                                                              |                                                                              aTCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGa                                                                             >  1:111115/1‑80 (MQ=255)   ccTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGa                                                                             >  1:111135/1‑78 (MQ=255)      gctgGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGAcg                                                                       >  2:111116/1‑81 (MQ=255)       ctgGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGAcgc                                                                      <  2:111075/81‑1 (MQ=255)             aaCCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGtt                                                                >  2:111102/1‑81 (MQ=255)                cTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGa                                                              >  1:111100/1‑80 (MQ=255)                cTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGAt                                                             <  2:111076/81‑1 (MQ=255)                 tGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATa                                                            >  1:111122/1‑81 (MQ=255)                  gggTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATAc                                                           >  1:111111/1‑81 (MQ=255)                        gCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACg                                                     >  2:111118/1‑81 (MQ=255)                        gCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACg                                                     >  1:111126/1‑81 (MQ=255)                          ccGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGgt                                                   <  1:111077/81‑1 (MQ=255)                          ccGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGgt                                                   >  2:111108/1‑81 (MQ=255)                           cGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGgtt                                                  >  1:111123/1‑81 (MQ=255)                            gATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGgttg                                                 >  2:111119/1‑81 (MQ=255)                               gCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTa                                              >  1:111121/1‑81 (MQ=255)                               gCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTa                                              >  2:111128/1‑81 (MQ=255)                                 aaacacaCCTGAAGCGGTAAAACGCAATCTGAAAAAATTTTTAAGAGACAGACGCGTGGTTGATACCTCACGGTTGTTAtg                                            <  1:994073/78‑1 (MQ=255)                                  cccACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTAtgg                                           >  2:111125/1‑81 (MQ=255)                                  cccACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTAtgg                                           <  1:111078/81‑1 (MQ=255)                                    cacaCCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTAtggtg                                         >  1:111112/1‑81 (MQ=255)                                        ccTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCa                                     <  1:111079/81‑1 (MQ=255)                                               cGGTAAAACGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATtgctgc                              <  2:111080/81‑1 (MQ=255)                                                   aaaaCGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCgcgg                          >  1:111132/1‑81 (MQ=255)                                                    aaaCGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCgcggc                         >  2:111114/1‑81 (MQ=255)                                                     aaCGCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCgcggcg                        >  1:111120/1‑81 (MQ=255)                                                        gCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGa                     <  2:111081/81‑1 (MQ=255)                                                        gCTATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGa                     <  1:111082/81‑1 (MQ=255)                                                          tATCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGAtt                   >  2:394037/1‑81 (MQ=255)                                                           aTCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGAttt                  >  1:111133/1‑81 (MQ=255)                                                           aTCTGAAACAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGAttt                  >  2:111138/1‑81 (MQ=255)                                                                 aaCAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGc            >  2:111149/1‑81 (MQ=255)                                                                  aCAATTTTTAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCt           <  2:111083/81‑1 (MQ=255)                                                                        tttAAGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTc     >  2:111143/1‑81 (MQ=255)                                                                         ttAAGTGACAGACGCGTGGTTGATAACTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGCGCTc     <  1:111084/80‑1 (MQ=255)                                                                           aaGTGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTcgcc  >  1:111167/1‑81 (MQ=255)                                                                              |                                                                              ATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCC  >  NC_000913/498076‑498230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑