Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,947	A→G	Y298C (TAT→TGT)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,947	0	A	G	100.0%	79.4 / NA	24	Y298C (TAT→TGT)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/17);  total (7/17)

ATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAG  >  NC_000913/498869‑499019                                                                               |                                                                         aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAatgta                                                                        >  1:111296/1‑81 (MQ=255)    gTCTGGAGACGCTGGAAGAGATTGCCGGGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTAtt                                                                      <  1:111241/80‑1 (MQ=255)            aCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCtt                                                             <  2:111243/81‑1 (MQ=255)            aCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCtt                                                             <  2:111242/81‑1 (MQ=255)              gCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTaa                                                           >  2:111322/1‑81 (MQ=255)               cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAAt                                                          >  2:111280/1‑81 (MQ=255)                  gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGcc                                                       <  2:111245/81‑1 (MQ=255)                  gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGc                                                        <  2:111246/80‑1 (MQ=255)                  gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGc                                                        <  2:111244/80‑1 (MQ=255)                   aaGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCa                                                      >  2:111286/1‑81 (MQ=255)                    agagATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCAc                                                     <  1:111247/81‑1 (MQ=255)                       gaTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGACGCTTAATGCCACGcc                                                  <  2:111248/81‑1 (MQ=255)                            ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAAc                                             <  2:111249/81‑1 (MQ=255)                                  aaaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATc                                        >  2:111284/1‑80 (MQ=255)                                         tGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatgat                                <  1:111250/81‑1 (MQ=255)                                          gAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatgatg                               >  1:111287/1‑81 (MQ=255)                                           aGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatgatg                               <  2:111251/80‑1 (MQ=255)                                           aGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATgg                              <  1:111252/81‑1 (MQ=255)                                            ggTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGc                             >  1:111282/1‑81 (MQ=255)                                                ttCCTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAAt                         >  1:111293/1‑81 (MQ=255)                                                   cTCGGTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCtt                      <  2:111253/81‑1 (MQ=255)                                                      ggTGCCGGCGGGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCttgt                    <  1:111254/80‑1 (MQ=255)                                                            ggcggGAAAAAATATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCcgcg             <  1:111255/81‑1 (MQ=255)                                                                      aaTATGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTaaa   <  1:111256/81‑1 (MQ=255)                                                                       atatGAATGTATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAg  <  1:614271/81‑1 (MQ=255)                                                                               |                                                                         ATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAG  >  NC_000913/498869‑499019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑