Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	508,288	A→G	D24G (GAT→GGT)	ybaQ →	putative DNA‑binding transcriptional regulator YbaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	508,288	0	A	G	100.0%	110.6 / NA	32	D24G (GAT→GGT)	ybaQ	putative DNA‑binding transcriptional regulator YbaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)

AAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGT  >  NC_000913/508212‑508364                                                                             |                                                                             aaaaCCATGGAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGttt                                                                           >  2:113147/1‑80 (MQ=255)     ccATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGaaaa                                                                      <  1:113125/81‑1 (MQ=255)      cATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAAt                                                                     >  2:113169/1‑81 (MQ=255)       aTGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATc                                                                    <  2:113126/81‑1 (MQ=255)                  aacaaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAg                                                          >  1:113157/1‑80 (MQ=255)                       gAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGt                                                    >  2:113168/1‑81 (MQ=255)                        aaaaCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGtt                                                   <  1:113127/81‑1 (MQ=255)                            ccGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTtgctg                                               <  2:113128/81‑1 (MQ=255)                              gacgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCa                                             >  1:113178/1‑81 (MQ=255)                              gacgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCa                                             >  1:113174/1‑81 (MQ=255)                                cgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATg                                           >  2:113161/1‑81 (MQ=255)                                 gacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGt                                          >  1:113171/1‑81 (MQ=255)                                   cacCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTc                                        <  2:113129/81‑1 (MQ=255)                                    acCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCa                                       <  1:113130/81‑1 (MQ=255)                                      ccGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATc                                     <  1:113131/81‑1 (MQ=255)                                       cGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCg                                    >  1:113155/1‑81 (MQ=255)                                         gagaTATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGt                                   >  1:113159/1‑80 (MQ=255)                                          agaTATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTaa                                 <  1:113132/81‑1 (MQ=255)                                               ttcttcTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGc                             <  1:113133/80‑1 (MQ=255)                                                        aTGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCAc                   >  2:113170/1‑81 (MQ=255)                                                         tGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACt                  <  2:113134/81‑1 (MQ=255)                                                           aaTATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGa                <  2:113135/81‑1 (MQ=255)                                                             tatCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGAt               >  1:113172/1‑80 (MQ=255)                                                                cTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCaa            >  2:113173/1‑80 (MQ=255)                                                                 tGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAAt           >  1:113180/1‑80 (MQ=255)                                                                  ggAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATaa         >  2:113185/1‑81 (MQ=255)                                                                   gAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATaa         <  1:113137/80‑1 (MQ=255)                                                                   gAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAAc        <  1:113136/81‑1 (MQ=255)                                                                     aCCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACaa      >  2:113167/1‑81 (MQ=255)                                                                      ccGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAAt     >  2:113163/1‑81 (MQ=255)                                                                        gCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCg   >  1:113166/1‑81 (MQ=255)                                                                         cTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGt  <  2:113138/81‑1 (MQ=255)                                                                             |                                                                             AAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGT  >  NC_000913/508212‑508364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑