Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	538,029	A→G	I133V (ATT→GTT)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	538,029	0	A	G	100.0%	59.2 / NA	19	I133V (ATT→GTT)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/11);  total (8/11)

CGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCT  >  NC_000913/537953‑538099                                                                             |                                                                       cGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCt                                                                    <  2:119261/81‑1 (MQ=255)        ttaAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTgg                                                              <  2:119263/80‑1 (MQ=255)        ttaAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTgg                                                              >  1:119303/1‑80 (MQ=255)        ttaAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGc                                                             <  1:119262/81‑1 (MQ=255)          aaGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCa                                                            >  2:119290/1‑80 (MQ=255)                       gccgccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGa                                              >  2:119294/1‑81 (MQ=255)                             atcatGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAttttt                                         >  2:119298/1‑80 (MQ=255)                                atGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAAct                                     <  2:119264/81‑1 (MQ=255)                                atGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAAc                                      <  1:119265/80‑1 (MQ=255)                                         ggtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtggt                            >  1:119297/1‑81 (MQ=255)                                          gtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtggt                            <  2:119266/80‑1 (MQ=255)                                               cAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGAttt                       <  2:119267/80‑1 (MQ=255)                                                    ttACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCActct                 >  1:119291/1‑81 (MQ=255)                                                     tACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTg                >  2:119296/1‑81 (MQ=255)                                                       cgcgGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGtt              <  1:119268/81‑1 (MQ=255)                                                       cgcgGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGt               <  1:119269/80‑1 (MQ=255)                                                       cgcgGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGt               >  2:119295/1‑80 (MQ=255)                                                             gTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCt        <  2:119270/81‑1 (MQ=255)                                                                    gCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTctct  <  2:119271/80‑1 (MQ=255)                                                                             |                                                                       CGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCT  >  NC_000913/537953‑538099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑