Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,434	G→A	L96L (CTG→CTA)	allB →	allantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,434	0	G	A	100.0%	134.3 / NA	37	L96L (CTG→CTA)	allB	allantoinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/19);  total (18/19)

GGAAGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTGCCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACA  >  NC_000913/539356‑539511                                                                               |                                                                              ggAAGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGctacc                                                                             >  2:119516/1‑81 (MQ=255)  gAAGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGctacct                                                                            <  2:119471/81‑1 (MQ=255)   aaGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGctacctg                                                                           <  1:119472/81‑1 (MQ=255)    aGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGctacctgc                                                                          <  1:119473/81‑1 (MQ=255)       ttATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAAc                                                                       >  1:119512/1‑81 (MQ=255)          tGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGt                                                                    <  1:119474/81‑1 (MQ=255)              aCCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGAc                                                                >  1:119514/1‑81 (MQ=255)               ccGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGAcc                                                               >  1:119519/1‑81 (MQ=255)                cGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACcg                                                              >  2:119517/1‑81 (MQ=255)                 ggTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACcgc                                                             <  1:119475/81‑1 (MQ=255)                         cgcAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAAt                                                     <  1:119476/81‑1 (MQ=255)                            aGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTg                                                   >  1:119532/1‑80 (MQ=255)                             gCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGaa                                                 >  1:119531/1‑81 (MQ=255)                                   aaaGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAg                                           <  2:119478/81‑1 (MQ=255)                                   aaaGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAg                                           <  2:119477/81‑1 (MQ=255)                                      ggtggtATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTc                                        >  1:119545/1‑81 (MQ=255)                                      ggtggtATCACCACCATGATCGAAATGCCGCACAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTc                                        <  2:119479/81‑1 (MQ=255)                                             tCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGcc                                  <  2:119480/80‑1 (MQ=255)                                              caccacCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGc                                >  2:119535/1‑81 (MQ=255)                                               accaccATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGc                                >  1:119571/1‑80 (MQ=255)                                               accaccATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCt                               <  1:119481/81‑1 (MQ=255)                                                ccaccaTGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTa                              <  2:119482/81‑1 (MQ=255)                                                 caccaTGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTaa                             <  1:119483/81‑1 (MQ=255)                                                  accaTGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTa                              >  1:119523/1‑79 (MQ=255)                                                    caTGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAgg                          >  1:119528/1‑81 (MQ=255)                                                      tGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCa                        <  2:631184/81‑1 (MQ=255)                                                        aTCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCaa                       <  1:119484/80‑1 (MQ=255)                                                        aTCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAg                      >  1:119543/1‑81 (MQ=255)                                                        aTCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAg                      >  1:119530/1‑81 (MQ=255)                                                        aTCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAg                      >  2:119556/1‑81 (MQ=255)                                                                gCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTAt              >  1:119527/1‑81 (MQ=255)                                                                 ccGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTAtt             <  1:119485/81‑1 (MQ=255)                                                                 ccGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTAtt             >  1:119525/1‑81 (MQ=255)                                                                 ccGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTAt              <  2:119486/80‑1 (MQ=255)                                                                  cGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTg            <  2:119487/81‑1 (MQ=255)                                                                   gCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGa           >  1:119551/1‑81 (MQ=255)                                                                      cAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGc        >  1:119537/1‑81 (MQ=255)                                                                       aaCCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATg         <  1:119488/79‑1 (MQ=255)                                                                        aCCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCgg      <  1:119489/81‑1 (MQ=255)                                                                          cAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGca    <  1:119490/81‑1 (MQ=255)                                                                            gCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGcaca  <  2:119491/81‑1 (MQ=255)                                                                               |                                                                              GGAAGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTGCCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACA  >  NC_000913/539356‑539511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑