Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	553,240	T→C	E234G (GAG→GGG)	lpxH ←	UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	553,240	0	T	C	100.0%	79.1 / NA	24	E234G (GAG→GGG)	lpxH	UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/8);  total (16/8)

GAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTG  >  NC_000913/553165‑553316                                                                            |                                                                             gagagCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACg                                                                         >  1:122455/1‑81 (MQ=255)    agCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCa                                                                      >  1:122469/1‑81 (MQ=255)       aaGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATcc                                                                   <  1:122430/81‑1 (MQ=255)       aaGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATcc                                                                   >  2:122459/1‑81 (MQ=255)       aaGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATcc                                                                   <  2:122429/81‑1 (MQ=255)       aaGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATcc                                                                   >  1:122460/1‑81 (MQ=255)       aaGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATcc                                                                   <  1:122428/81‑1 (MQ=255)        aGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATccg                                                                  <  1:122431/81‑1 (MQ=255)          gAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATccgcc                                                                <  2:122432/81‑1 (MQ=255)           aaaaCGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATccgccg                                                               >  1:122463/1‑81 (MQ=255)           aaaaCGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATccgccg                                                               >  2:122461/1‑81 (MQ=255)              aCGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGa                                                            >  1:122453/1‑81 (MQ=255)                          cTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCAtt                                                 >  2:122475/1‑80 (MQ=255)                               aaaTCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCt                                           >  2:122466/1‑81 (MQ=255)                                     gCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTa                                     >  2:122513/1‑81 (MQ=255)                                         aGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGcc                                 <  2:122433/81‑1 (MQ=255)                                           ttGCGGGTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                               >  1:122465/1‑81 (MQ=255)                                                 gTTTTTTAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCAccc                         <  2:122434/81‑1 (MQ=255)                                                  ttttttAAAACGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCa                        <  2:122435/81‑1 (MQ=255)                                                           aCGGAAAATGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCTAGTACCAcgc               >  1:122468/1‑81 (MQ=255)                                                               aaaaTGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGaa            >  2:122473/1‑80 (MQ=255)                                                                 aaTGAATCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAg         >  2:122478/1‑81 (MQ=255)                                                                      aTCAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGt    >  1:122485/1‑81 (MQ=255)                                                                        cAGCCCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTg  >  1:122479/1‑81 (MQ=255)                                                                            |                                                                             GAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTG  >  NC_000913/553165‑553316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑