Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	560,244	A→G	N183S (AAT→AGT)	sfmD →	putative fimbrial usher protein SfmD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	560,244	0	A	G	100.0%	73.9 / NA	22	N183S (AAT→AGT)	sfmD	putative fimbrial usher protein SfmD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/8);  total (14/8)

GTTGTTGAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAATTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGG  >  NC_000913/560173‑560319                                                                        |                                                                            gttgttGAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAAttt                                                                    >  2:123800/1‑81 (MQ=255)       gAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCgg                                                              >  1:123805/1‑81 (MQ=255)         gTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTa                                                            <  1:123775/81‑1 (MQ=255)            gcgcTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAAc                                                          <  2:123776/80‑1 (MQ=255)                 cACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAgg                                                     >  2:123836/1‑80 (MQ=255)                     gTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATg                                                >  2:123802/1‑81 (MQ=255)                      ttACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATgg                                               >  2:123808/1‑81 (MQ=255)                           ttCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACg                                          <  2:123777/81‑1 (MQ=255)                              cGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAACTTCACCGGTAACAGAGGTAATGGTAACGa                                         >  2:123804/1‑79 (MQ=255)                               gCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAg                                      >  2:123810/1‑81 (MQ=255)                                 cTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGc                                     >  2:123819/1‑80 (MQ=255)                                 cTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCt                                    <  2:123778/81‑1 (MQ=255)                                   gAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTAt                                  >  1:123799/1‑81 (MQ=255)                                   gAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTAt                                  >  2:123815/1‑81 (MQ=255)                                       agTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTAttttt                              <  2:123779/81‑1 (MQ=255)                                           ggATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAg                          >  1:123801/1‑81 (MQ=255)                                             aTGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGt                         >  2:123811/1‑80 (MQ=255)                                                  ggTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCt                    >  1:123809/1‑80 (MQ=255)                                                   gTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCa                  <  1:123780/81‑1 (MQ=255)                                                    tATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAg                 <  2:123781/81‑1 (MQ=255)                                                            cTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGAtt          >  1:123807/1‑80 (MQ=255)                                                                    cTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTgg  <  1:123782/80‑1 (MQ=255)                                                                        |                                                                            GTTGTTGAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAATTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGG  >  NC_000913/560173‑560319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑