Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	568,797	T→C	Y35H (TAT→CAT)	ylcJ →	protein YlcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	568,797	0	T	C	100.0%	22.5 / NA	8	Y35H (TAT→CAT)	ylcJ	protein YlcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/4);  total (4/4)

ATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCCCACCA  >  NC_000913/568722‑568864                                                                            |                                                                    aTGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATAtt                                                                >  1:125359/1‑81 (MQ=255)                 tGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGAcc                                                 <  1:125322/80‑1 (MQ=255)                    aTTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTaa                                             >  2:125335/1‑81 (MQ=255)                            gtgCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCtt                                      <  1:125323/80‑1 (MQ=255)                                      gATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTtaaataaa                            >  2:125336/1‑80 (MQ=255)                                                     aaaTTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATta            <  1:125324/81‑1 (MQ=255)                                                              tACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCccacc   <  1:125325/81‑1 (MQ=255)                                                               aCGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCccacca  >  2:125334/1‑81 (MQ=255)                                                                            |                                                                    ATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCCCACCA  >  NC_000913/568722‑568864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑