Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	570,248	A→G	L449L (TTA→TTG)	ybcK →	DLP12 prophage; putative recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	570,248	0	A	G	100.0%	44.0 / NA	14	L449L (TTA→TTG)	ybcK	DLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/9);  total (5/9)

ACTTTGAAGAGTAAAGGTGTAAATTCATTCAGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTATGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGATAATAAAACCTGTGATATC  >  NC_000913/570171‑570317                                                                              |                                                                      aCTTTGAAGAGTAAAGGTGTAAATTCATTCAGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTGTGc                                                                    <  1:125463/81‑1 (MQ=255)  cTTTGAAGAGTAAAGGTGTAAATTCATTCAGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTGTGcc                                                                   <  2:125464/81‑1 (MQ=255)   tttGAAGAGTAAAGGTGTAAATTCATTCAGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTGTGCCg                                                                  >  1:125485/1‑81 (MQ=255)              aaGGTGTAAATTCATTCAGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTGTGCCGTACACTTGcc                                                        <  1:125466/80‑1 (MQ=255)              aaGGTGTAAATTCATTCAGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTGTGCCGTACACTTGCCt                                                       <  1:125465/81‑1 (MQ=255)                            ttcaGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGa                                          >  2:125491/1‑80 (MQ=255)                                      tttATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCAtaa                               >  1:125493/1‑81 (MQ=255)                                               ttGACTTAACCAGTAAAAATGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATAc                        <  2:125467/79‑1 (MQ=255)                                                 gACTTAACCAGTAAAAATGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGa                     >  2:125497/1‑80 (MQ=255)                                                              aaaaTGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGATAATAAAACCtgtg       <  2:688861/81‑1 (MQ=255)                                                              aaaaTGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGATAATAAAACCtgt        <  1:125468/80‑1 (MQ=255)                                                                aaTGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGATAATAAAACCTGTGat     <  2:125469/81‑1 (MQ=255)                                                                 aTGGACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGATAATAAAACCTGTGata    <  2:125470/81‑1 (MQ=255)                                                                   ggACGAGAGTTGTGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGATAATAAAACCTGTGATATc  >  1:125492/1‑81 (MQ=255)                                                                              |                                                                      ACTTTGAAGAGTAAAGGTGTAAATTCATTCAGTGATTTTTATGCTATTGACTTAACCAGTAAAAATGGACGAGAGTTATGCCGTACACTTGCCTATAAAACATTCGAAAAAATCATAATTAATACGGATAATAAAACCTGTGATATC  >  NC_000913/570171‑570317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑