Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	598,000	G→A	T174T (ACG→ACA)	cusB →	copper/silver export system membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	598,000	0	G	A	100.0%	68.3 / NA	20	T174T (ACG→ACA)	cusB	copper/silver export system membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

TTCTCGACCTGACCATTCCTGACTGGGTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGA  >  NC_000913/597927‑598073                                                                          |                                                                          ttCTCGACCTGACCATTCCTGACTGGGTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGAcc                                                                     >  2:130244/1‑80 (MQ=255)    tcGACCTGACCATTCCTGACTGGGTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGa                                                                 >  2:130250/1‑81 (MQ=255)    tcGACCTGACCATTCCTGACTGGGTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGa                                                                 >  1:130253/1‑81 (MQ=255)                ttCCTGACTGGGTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTc                                                     <  1:130221/81‑1 (MQ=255)                       cTGGGTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCg                                              >  1:130271/1‑81 (MQ=255)                          ggTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGTGCGGCt                                           <  2:130222/81‑1 (MQ=255)                          ggTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGc                                            <  1:130223/80‑1 (MQ=255)                             ggAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGc                                         >  2:130257/1‑80 (MQ=255)                                aGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACt                                     >  1:130262/1‑81 (MQ=255)                                         tgagtATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAAt                            <  2:130224/81‑1 (MQ=255)                                            gtATTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGcc                         <  1:130225/81‑1 (MQ=255)                                              aTTTACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCg                        >  2:130252/1‑80 (MQ=255)                                                 tACTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAg                     <  2:130226/80‑1 (MQ=255)                                                  aCTGCTGCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGc                   >  2:130259/1‑81 (MQ=255)                                                   ctgctgCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGc                   <  2:130227/80‑1 (MQ=255)                                                   ctgctgCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGc                   <  2:130228/80‑1 (MQ=255)                                                   ctgctgCGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCg                  <  1:130229/81‑1 (MQ=255)                                                      ctgcGCGAAACCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCgg                 <  2:130230/79‑1 (MQ=255)                                                             aaaCCGGCGGTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTcgcc        <  1:130231/81‑1 (MQ=255)                                                                   gcggTACAGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGa  >  1:130263/1‑81 (MQ=255)                                                                          |                                                                          TTCTCGACCTGACCATTCCTGACTGGGTGGAAGCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGA  >  NC_000913/597927‑598073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑