| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 600,325 | A→G | T538A (ACC→GCC) | cusA → | copper/silver export system RND permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 600,325 | 0 | A | G | 97.3% | 119.2 / ‑3.9 | 37 | T538A (ACC→GCC) | cusA | copper/silver export system RND permease |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (17/19); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACC > NC_000913/600251‑600401 | gCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACgc < 1:130741/81‑1 (MQ=255)gCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACgc < 2:130740/81‑1 (MQ=255) aGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACgctg < 1:130742/81‑1 (MQ=255) aGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACgctg > 1:130790/1‑81 (MQ=255) aaCCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGctgctg > 2:130788/1‑81 (MQ=255) gCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTggc > 2:130787/1‑81 (MQ=255) aaTCGTCTTTTGATTCGTGTTTAACATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGgcggcg > 2:130794/1‑81 (MQ=255) aaTCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTggcggc < 1:130743/80‑1 (MQ=255) gCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCttt < 1:130744/81‑1 (MQ=255) gCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCtt < 1:130745/80‑1 (MQ=255) cTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTc > 2:130762/1‑81 (MQ=255) cTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTc < 2:130746/81‑1 (MQ=255) aTTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCt < 1:130747/80‑1 (MQ=255) cGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGAc < 2:130748/80‑1 (MQ=255) gtgtTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACgg > 2:130798/1‑81 (MQ=255) tttATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGtt < 2:130749/80‑1 (MQ=255) tATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTtctc > 1:130791/1‑81 (MQ=255) tATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTtct < 2:130750/80‑1 (MQ=255) tATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTtct < 2:130751/80‑1 (MQ=255) tcatcCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTg < 1:130752/81‑1 (MQ=255) tcatcCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTg > 2:130780/1‑81 (MQ=255) cGCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCg < 2:130753/80‑1 (MQ=255) gCTGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCt > 1:130797/1‑81 (MQ=255) tGTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTc > 2:130805/1‑80 (MQ=255) gTTGCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCaa > 1:130792/1‑81 (MQ=255) gCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATa > 1:130793/1‑80 (MQ=255) gCTGAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATa < 2:130754/80‑1 (MQ=255) gAAAGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGt > 1:130786/1‑81 (MQ=255) aGTACTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTgg > 1:130773/1‑81 (MQ=255) cTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCggg < 2:130756/81‑1 (MQ=255) cTGCACTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCgg < 2:130755/80‑1 (MQ=255) aCTGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAAt > 2:130800/1‑81 (MQ=255) tGGCCGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAAttt > 2:130796/1‑81 (MQ=255) cGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTAc < 1:130757/81‑1 (MQ=255) cGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTAc < 2:130758/81‑1 (MQ=255) cGAAAGCCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTAc > 2:130801/1‑81 (MQ=255) gAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGATGACGGTTCTCTGGCGGCTCAATCAAGTTGACAGGGAATTTTTaac > 2:1001961/1‑79 (MQ=255) | GCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACC > NC_000913/600251‑600401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |