Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,771	Δ1 bp	intergenic (+147/‑167)	asnT → / → yeeJ	tRNA‑Asn/inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,768	0	C	.	100.0%	77.4 / NA	18	intergenic (+144/‑170)	asnT/yeeJ	tRNA‑Asn/inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (7/11);  total (7/11)

AGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTT  >  NC_000913/2044692‑2044845                                                                             |                                                                              aGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccctc                                                                           >  1:336380/1‑79 (MQ=255) aGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccctc                                                                           >  1:336385/1‑79 (MQ=255)  gCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccctc                                                                           <  1:336354/80‑3 (MQ=255)  gCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccctc                                                                           >  2:336382/1‑78 (MQ=255)  gCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccctc                                                                           <  2:336353/80‑3 (MQ=255)  gCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccctc                                                                           <  1:336352/80‑3 (MQ=255)     ggTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGAtt                                                                      <  2:336355/81‑1 (MQ=255)         tttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATtata                                                                   <  1:336356/80‑1 (MQ=255)            aaCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAAc                                                               >  1:336378/1‑81 (MQ=255)             aCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAAc                                                               <  1:336357/80‑1 (MQ=255)              cATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACtt                                                             >  2:336388/1‑81 (MQ=255)               aTCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTg                                                            <  2:336358/81‑1 (MQ=255)                              gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGtc                                             >  1:336391/1‑81 (MQ=255)                                ccTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGtctc                                           <  2:336359/81‑1 (MQ=255)                                     aTGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctc                                      >  2:336389/1‑81 (MQ=255)                                                 gTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTcaca                          <  1:336360/81‑1 (MQ=255)                                                  ttttGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCacac                         <  1:336361/81‑1 (MQ=255)                                                          tGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACt                 >  2:336386/1‑81 (MQ=255)                                                              cGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCt             <  1:336362/81‑1 (MQ=255)                                                               gCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTa            <  2:336363/81‑1 (MQ=255)                                                                  aTCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAAc          >  2:336392/1‑80 (MQ=255)                                                                   tCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACg         >  2:336383/1‑80 (MQ=255)                                                                     aCCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGaaa      <  1:336365/81‑1 (MQ=255)                                                                     aCCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGaaa      <  1:336364/81‑1 (MQ=255)                                                                         attttCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGt   <  1:336366/80‑1 (MQ=255)                                                                          ttttCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtt  <  2:336367/81‑1 (MQ=255)                                                                             |                                                                              AGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTT  >  NC_000913/2044692‑2044845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑