Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,632	Δ1 bp	coding (354/1947 nt)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,629	0	A	.	100.0%	83.0 / NA	19	coding (357/1947 nt)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/9);  total (10/9)

ACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTAC  >  NC_000913/2150555‑2150702                                                                           |                                                                          aCGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGaaatcc                                                                      >  1:352245/1‑77 (MQ=255)               gTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAggg                                                      >  1:352232/1‑81 (MQ=255)                     gTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAActc                                                >  1:352237/1‑81 (MQ=255)                     gTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAActc                                                >  1:352231/1‑81 (MQ=255)                        cTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTg                                              <  1:352210/80‑1 (MQ=255)                           gTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGc                                            <  1:352211/79‑1 (MQ=255)                                  gTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGAtgtg                                   >  1:352242/1‑81 (MQ=255)                                         aaGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCt                            <  1:352212/81‑1 (MQ=255)                                          aGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCta                           <  1:352213/81‑1 (MQ=255)                                          aGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCta                           >  1:352246/1‑81 (MQ=255)                                           gATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCtat                          <  2:352214/81‑1 (MQ=255)                                            aTGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCta                           >  2:352248/1‑79 (MQ=255)                                               aaGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCta                           >  1:352243/1‑76 (MQ=255)                                               aaGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGAt                      <  1:352215/81‑1 (MQ=255)                                                  cAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCat                   <  1:352216/81‑1 (MQ=255)                                                     tatTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATg                <  2:352217/81‑1 (MQ=255)                                                          cgcgcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCg           >  1:352218/1‑81 (MQ=255)                                                           gcgcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCgg          >  2:352239/1‑81 (MQ=255)                                                             gcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGtt        >  2:352247/1‑81 (MQ=255)                                                                   tAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTAc  <  1:352219/81‑1 (MQ=255)                                                                           |                                                                          ACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTAC  >  NC_000913/2150555‑2150702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑