Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,367	(A)5→4	coding (466/2190 nt)	pdeA ←	putative c‑di‑GMP phosphodiesterase PdeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,363	0	A	.	100.0%	120.5 / NA	27	coding (470/2190 nt)	pdeA	putative c‑di‑GMP phosphodiesterase PdeA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/17);  total (10/17)

ATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGCGT  >  NC_000913/2517291‑2517439                                                                         |                                                                             aTGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaaggtg                                                                       >  2:414252/1‑76 (MQ=255)    ttgtAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGata                                                                  >  1:414254/1‑81 (MQ=255)    ttgtAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGat                                                                   <  1:414216/80‑1 (MQ=255)       tAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATc                                                                <  1:414217/80‑1 (MQ=255)       tAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATc                                                                <  1:414218/80‑1 (MQ=255)       tAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCt                                                               <  1:414219/81‑1 (MQ=255)         aaTCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCtt                                                              <  2:414220/80‑1 (MQ=255)              gCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCgg                                                        <  2:414221/81‑1 (MQ=255)                acaGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGa                                                      <  1:414222/81‑1 (MQ=255)                  aGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGaaa                                                    >  2:414247/1‑81 (MQ=255)                     ggTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATc                                                  >  1:414258/1‑80 (MQ=255)                              gCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTc                                        <  1:414223/81‑1 (MQ=255)                                  aGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTcccc                                     <  2:414225/80‑1 (MQ=255)                                  aGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTccc                                      <  2:414224/79‑1 (MQ=255)                                  aGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCca                                    >  1:414256/1‑81 (MQ=255)                                        tCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGa                               >  1:414263/1‑80 (MQ=255)                                            cGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACAt                          <  1:414226/81‑1 (MQ=255)                                                cGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACAtgctg                      <  1:414227/81‑1 (MQ=255)                                                     aaaTGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCAttt                 >  1:414259/1‑81 (MQ=255)                                                           cATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGcc           <  1:414228/81‑1 (MQ=255)                                                              ccGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGAt        >  1:414253/1‑81 (MQ=255)                                                               cGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATc       >  1:414255/1‑81 (MQ=255)                                                               cGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATc       >  1:414251/1‑81 (MQ=255)                                                               cGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATc       <  2:414229/81‑1 (MQ=255)                                                               cGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATc       <  2:414230/81‑1 (MQ=255)                                                                gCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCg      <  1:414231/81‑1 (MQ=255)                                                                   tCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGCg   <  2:414232/81‑1 (MQ=255)                                                                    cGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGCGt  >  1:414260/1‑81 (MQ=255)                                                                         |                                                                             ATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGCGT  >  NC_000913/2517291‑2517439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑