Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,159	(A)6→5	coding (212/1260 nt)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,154	0	A	.	100.0%	31.1 / NA	8	coding (207/1260 nt)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/3);  total (5/3)

AAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAG  >  NC_000913/3797080‑3797227                                                                           |                                                                          aaaaTATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATaaaaac                                                                      <  1:686115/80‑2 (MQ=255)         taataTCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAttct                                                             <  1:686116/80‑1 (MQ=255)                aCTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCttt                                                     >  2:686123/1‑81 (MQ=255)                       taGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATAtt                                              >  2:686132/1‑81 (MQ=255)                                      cACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGgcttgct                               >  1:686127/1‑81 (MQ=255)                                        cTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGcttgcttg                             <  2:686117/81‑1 (MQ=255)                                                              aaaaTTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGtt       >  2:686124/1‑81 (MQ=255)                                                              aaaaTTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGtt       >  2:686126/1‑81 (MQ=255)                                                                aaTTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTa     <  1:686118/81‑1 (MQ=255)                                                                      aatat‑aaAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAg  >  2:686125/1‑78 (MQ=255)                                                                           |                                                                          AAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAG  >  NC_000913/3797080‑3797227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑