Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,442,102	(T)5→4	coding (742/990 nt)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,442,098	0	T	.	100.0%	78.4 / NA	18	coding (746/990 nt)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/8);  total (10/8)

GATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTGGTATTGACGATCATCACGCCATTTT  >  NC_000913/1442029‑1442173                                                                      |                                                                            gATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCtt                                                                 <  1:253744/81‑1 (MQ=255) gATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCtt                                                                 <  1:253743/81‑1 (MQ=255)             aaGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCt                                                     >  2:253763/1‑81 (MQ=255)                   aGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGaat                                               >  2:253764/1‑81 (MQ=255)                     aTCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGaatca                                             <  1:253745/81‑1 (MQ=255)                     aTCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGaatc                                              >  2:253767/1‑80 (MQ=255)                      tCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGaatcaa                                            >  1:253770/1‑81 (MQ=255)                         cgttcgttCTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGaatcaatc                                          >  1:253772/1‑80 (MQ=255)                          gttcgttcTCATACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAAtcaatcaa                                        >  2:253762/1‑81 (MQ=255)                                    aTACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACcgc                                <  2:253746/79‑1 (MQ=255)                                    aTACACGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGa                              >  2:253768/1‑81 (MQ=255)                                       cacGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACt                            <  1:253748/80‑1 (MQ=255)                                       cacGTCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTg                           <  1:253747/81‑1 (MQ=255)                                           tCCATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTGGTAt                       >  1:253777/1‑81 (MQ=255)                                             cATACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTGGTAtt                      <  1:253749/80‑1 (MQ=255)                                              aTACCCAACGAACCAATTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTGGTATTGa                    >  2:253765/1‑81 (MQ=255)                                                            aaTTTTCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTGGTATTGACGATCATCACGCCa      <  2:253750/81‑1 (MQ=255)                                                                ttCTGA‑TTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTGGTATTGACGATCATCACGCCAtttt  >  2:253778/1‑81 (MQ=255)                                                                      |                                                                            GATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGCTGCCTGAGAATCAATCAATGCACCGCGACTGGTATTGACGATCATCACGCCATTTT  >  NC_000913/1442029‑1442173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑