Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,490,954	(T)5→4	coding (53/531 nt)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,490,950	0	T	.	100.0%	72.3 / NA	17	coding (49/531 nt)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/6);  total (11/6)

TTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAA  >  NC_000913/1490885‑1491026                                                                  |                                                                             ttAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑CTTTACTGGTTATCg                                                               <  1:260664/80‑1 (MQ=255)        aGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTAt                                                       <  1:260665/81‑1 (MQ=255)        aGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTAt                                                       >  1:260674/1‑81 (MQ=255)          gAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTg                                                     >  2:260689/1‑81 (MQ=255)                    ggAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAg                                           >  1:260693/1‑81 (MQ=255)                     gAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGt                                          <  1:260666/81‑1 (MQ=255)                             atatTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGt                                  <  1:260667/81‑1 (MQ=255)                                      gTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCa                         >  2:260684/1‑81 (MQ=255)                                        tACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACg                       >  1:260695/1‑81 (MQ=255)                                                aGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATc               <  2:260668/81‑1 (MQ=255)                                                  cATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCgg             >  2:260690/1‑81 (MQ=255)                                                   aTTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGc            >  2:260687/1‑81 (MQ=255)                                                    ttCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGcc           <  1:260669/81‑1 (MQ=255)                                                      cACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCAc         >  2:260700/1‑81 (MQ=255)                                                        ctggctggTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACt        >  1:260694/1‑80 (MQ=255)                                                        ctggctggTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACt        >  2:260688/1‑80 (MQ=255)                                                             tggTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCaa  >  2:260697/1‑81 (MQ=255)                                                                  |                                                                             TTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAA  >  NC_000913/1490885‑1491026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑