Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,177	(T)6→5	coding (358/699 nt)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,172	0	T	.	100.0%	96.3 / NA	22	coding (363/699 nt)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/8);  total (14/8)

GGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGC  >  NC_000913/1915093‑1915244                                                                                |                                                                         ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtt                                                                         <  1:317098/81‑1 (MQ=255)     tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttc                                                                     <  2:317099/81‑2 (MQ=255)        cgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttcgct                                                                  <  2:317100/81‑5 (MQ=255)           aGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGc                                                              >  2:317124/1‑81 (MQ=255)               ggACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                          >  2:317128/1‑81 (MQ=255)                gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                          >  2:317132/1‑80 (MQ=255)                  cGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAg                                                       <  1:317101/81‑1 (MQ=255)                   gTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGc                                                      <  2:317102/81‑1 (MQ=255)                    tGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCa                                                     <  2:317103/81‑1 (MQ=255)                               cgcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCt                                           <  1:317104/80‑1 (MQ=255)                                gcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                                          >  2:317134/1‑80 (MQ=255)                                gcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                                          <  1:317105/80‑1 (MQ=255)                                 cggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                        >  2:317136/1‑81 (MQ=255)                                 cggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                        <  1:317106/81‑1 (MQ=255)                                  ggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                        >  1:317144/1‑80 (MQ=255)                                           cGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCttt                              >  1:317138/1‑81 (MQ=255)                                            gTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                             >  1:317135/1‑81 (MQ=255)                                             tcttctTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg                            <  1:317107/81‑1 (MQ=255)                                             tcttctTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg                            >  2:317133/1‑81 (MQ=255)                                                       cACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAg                  >  1:317150/1‑81 (MQ=255)                                                              agTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTg           >  2:317149/1‑81 (MQ=255)                                                               gTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc          <  1:317108/81‑1 (MQ=255)                                                                   cGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAg       >  2:317140/1‑80 (MQ=255)                                                                    ggCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCa     >  2:317141/1‑81 (MQ=255)                                                                       aGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGc  >  2:317109/1‑81 (MQ=255)                                                                                |                                                                         GGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGC  >  NC_000913/1915093‑1915244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑