| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,184,476 | (A)7→8 | intergenic (‑10/‑364) | ribB ← / → ubiK | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,184,469 | 1 | . | A | 94.1% | 58.2 / ‑2.7 | 17 | intergenic (‑3/‑371) | ribB/ubiK | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (7/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGT‑A‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGC > NC_000913/3184391‑3184534 | cTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGT‑a‑a < 2:545846/81‑1 (MQ=255) ttCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGT‑a‑a > 1:545879/1‑80 (MQ=255) aCGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGT‑a‑aaaa < 2:545847/80‑1 (MQ=255) agcgcagcCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGT‑a‑aaaaaaacc > 2:545889/1‑78 (MQ=255) agcCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCAc > 1:545892/1‑80 (MQ=255) gcCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACt > 2:545862/1‑80 (MQ=255) ccAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTaa < 1:545848/81‑1 (MQ=255) cAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTaaa < 2:545849/81‑1 (MQ=255) cGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGaa < 2:545850/81‑1 (MQ=255) gTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAAt < 2:545851/81‑1 (MQ=255) ggCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAg > 1:545886/1‑81 (MQ=255) gCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGt < 1:545852/81‑1 (MQ=255) aCCAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCt > 2:545881/1‑79 (MQ=255) ccAAAAGAGGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTa < 1:545853/80‑1 (MQ=255) agGAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTggc < 1:545854/81‑1 (MQ=255) agGAAAGTAGCGTCTGATTCATGGT‑ATAAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTggc < 2:545855/81‑1 (MQ=255) ggAAAGTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTggcg > 2:545888/1‑81 (MQ=255) gTAGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCata < 1:545856/81‑1 (MQ=255) aGCGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCata > 1:545887/1‑79 (MQ=255) cGTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGc < 2:545857/81‑1 (MQ=255) gTCTGATTCATGGTAA‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGc > 1:545900/1‑80 (MQ=255) | CTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGT‑A‑AAAAAACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGC > NC_000913/3184391‑3184534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |