| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 272,357 | 0 | G | T | 69.2% | 28.3 / 24.7 | 26 | intergenic (+167/‑223) | insN/eyeA | CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA |
| Reads supporting (aligned to +/- strand): ref base G (4/4); new base T (8/10); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACAT > NC_000913/272279‑272433 | ccAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCctct > 2:57003/1‑81 (MQ=255)ccAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCt < 2:56964/81‑1 (MQ=255) aaTCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACaa > 1:57011/1‑81 (MQ=255) tCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACg > 1:57001/1‑81 (MQ=255) ccACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACgg < 2:56965/80‑1 (MQ=255) ccGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGAt < 1:56966/81‑1 (MQ=255) ccGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGAt < 2:56967/81‑1 (MQ=255) cGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGAtt < 2:56968/81‑1 (MQ=255) gggTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTg < 2:56969/81‑1 (MQ=255) gtgACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGc > 1:57006/1‑81 (MQ=255) gACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGc > 2:56994/1‑79 (MQ=255) ggTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGc > 1:57012/1‑76 (MQ=255) ggAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCAc > 1:57025/1‑81 (MQ=255) gagaACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACt < 1:56970/81‑1 (MQ=255) aaCCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAg < 2:56971/81‑1 (MQ=255) ccTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGcc < 1:56972/81‑1 (MQ=255) ggCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAgatga < 2:56973/81‑1 (MQ=255) ggCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAgatga < 2:56974/81‑1 (MQ=255) gCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAgatgat < 2:56975/81‑1 (MQ=255) aGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATcc > 1:57008/1‑81 (MQ=255) aGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATcc > 2:57014/1‑81 (MQ=255) cGGCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGtt < 1:56976/80‑1 (MQ=255) ggCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGtt > 1:57019/1‑79 (MQ=255) ggCATGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTaa < 1:56977/81‑1 (MQ=255) aTGCCCGCAGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTa > 2:57013/1‑81 (MQ=255) aGGGTGATGTAACCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAgg > 2:57022/1‑81 (MQ=255) aCCCTCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACAt > 1:57010/1‑81 (MQ=255) | CCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACAT > NC_000913/272279‑272433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |