| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,253,781 | 0 | A | G | 72.2% | 22.9 / 10.0 | 18 | G724G (GGT→GGC) | ycgV | putative autotransporter adhesin YcgV |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base G (4/9); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCC > NC_000913/1253704‑1253855 | taGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCccac < 1:226839/79‑1 (MQ=255) tCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCGCCAAAc < 1:226840/81‑1 (MQ=255) cGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACt < 1:226841/80‑1 (MQ=255) tGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCGCCAAACTGGATAcc < 2:226842/81‑1 (MQ=255) tGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATAcc < 2:226843/81‑1 (MQ=255) cATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCGCCAAACTGGATACCGc > 2:226863/1‑81 (MQ=255) tgtTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGt < 2:226844/80‑1 (MQ=255) ccAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCGCCAAACTGGATACCGCTGTAACCCATg < 2:226845/81‑1 (MQ=255) aaTATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTc < 2:226846/81‑1 (MQ=255) cGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCa < 2:226847/81‑1 (MQ=255) cATACAACGGCATTACATCAGAGAGACGTTTATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGtt < 1:226848/81‑1 (MQ=255) aTACAACGGCATTACATCAGAGAGACGTTTATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGttt < 1:226849/81‑1 (MQ=255) gCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTATCCCATGTCAAAGCCGCTCAGTTTGCCACTgg > 2:226865/1‑81 (MQ=255) aTTACATCAGAGAGACGTTTATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCa < 1:226850/81‑1 (MQ=255) tACATCAGAGAGACGTTTATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCa > 1:226864/1‑79 (MQ=255) gTTTATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTc < 1:226851/81‑1 (MQ=255) tttATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTcc > 1:226861/1‑81 (MQ=255) tATCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTcccc > 1:226872/1‑81 (MQ=255) tCCCCGCCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTccccc < 2:226852/80‑1 (MQ=255) | TAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCC > NC_000913/1253704‑1253855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |