| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,376,140 | 0 | T | G | 66.7% | 35.6 / 19.1 | 24 | L36R (CTG→CGG) | ycjR | putative ketohexose‑3‑epimerase YcjR |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base G (14/2); total (21/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCT > NC_000913/1376063‑1376217 | cTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAAcggct > 2:244675/1‑81 (MQ=255) ttCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACGGCTgg > 1:244680/1‑81 (MQ=255) tCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAAcggct < 1:244648/78‑1 (MQ=255) tCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGt > 1:244670/1‑81 (MQ=255) aaaaCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGTTaac > 1:244669/1‑80 (MQ=255) tGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGTTAACAACATCg > 2:244672/1‑78 (MQ=255) tGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATCGACGGCAAACGGCTGGTTAACAACATCg > 2:244703/1‑78 (MQ=255) agaAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGTTAACAACATCGaagaag > 1:244684/1‑81 (MQ=255) aTTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCa > 2:244695/1‑80 (MQ=255) ttCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGTTAACAACATCGAAGAAGTCAAAg > 1:244682/1‑81 (MQ=255) tCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAgc > 1:244677/1‑81 (MQ=255) gTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGTAAACGGCTGGTTAACAACATCGAAGAAGTCAAAgcgg > 1:244693/1‑81 (MQ=255) gTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAgcgg > 1:244683/1‑81 (MQ=255) gagaTGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGa > 2:244696/1‑80 (MQ=255) agaTGGGCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGaaa > 2:244688/1‑81 (MQ=255) aTGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAAcc > 1:244673/1‑81 (MQ=255) gggCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCgg > 2:244698/1‑81 (MQ=255) gCTTCGATGGTTTTGAGATTGACGGCAAACGGCTGGTTAACAACATCGATGAAGTCAAAGCGGCGATCAAAGAAACCGGtt > 2:244689/1‑81 (MQ=255) gATGGTTTTGAGATTGACGGCAAACGGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCg > 1:244681/1‑81 (MQ=255) gATGGTTTTGAGATTGACGGCAAACGGCTGGATAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCg < 2:244649/81‑1 (MQ=255) aTGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCgg > 2:244694/1‑81 (MQ=255) agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCg > 1:244691/1‑81 (MQ=255) agaTTGACGGCAAACGGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCg > 1:244687/1‑81 (MQ=255) gCAAACGGCTGCTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTg > 1:244704/1‑81 (MQ=255) aaCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCt < 2:244650/81‑1 (MQ=255) aaCGGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCt < 1:244651/81‑1 (MQ=255) | CTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCT > NC_000913/1376063‑1376217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |