| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,832,908 | 0 | A | G | 65.4% | 19.4 / 24.5 | 26 | I161V (ATC→GTC) | xthA | exodeoxyribonuclease III |
| Reads supporting (aligned to +/- strand): ref base A (7/2); new base G (8/9); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATG > NC_000913/1832830‑1832988 | tACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTc > 1:305538/1‑81 (MQ=255) ccTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTc < 2:305513/79‑1 (MQ=255) ccTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCg < 1:305514/80‑1 (MQ=255) gAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCAtt > 1:305545/1‑81 (MQ=255) aaaCCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTg < 1:305516/81‑1 (MQ=255) aaaCCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTg < 1:305515/81‑1 (MQ=255) gAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCgaa < 1:305517/81‑1 (MQ=255) tCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCgaagaa > 2:305535/1‑80 (MQ=255) aaaCGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCGAAGAAAAc > 2:305548/1‑81 (MQ=255) tGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGt > 2:305565/1‑79 (MQ=255) aTAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAgc > 2:305549/1‑81 (MQ=255) tAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAgcg > 1:305532/1‑81 (MQ=255) aTCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAgcgc < 2:305519/80‑1 (MQ=255) aTCCGGTACTGATTATGGGCGATATGAATATCAGACCTACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAGCgct < 1:305518/81‑1 (MQ=255) ttATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCg < 2:305521/81‑1 (MQ=255) ttATGGGCGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCg < 2:305520/81‑1 (MQ=255) tATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCgg > 2:305537/1‑81 (MQ=255) aTGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCgg > 1:305544/1‑80 (MQ=255) cGATATGAATATCAGCCCTACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATg > 2:305539/1‑81 (MQ=255) atGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGctct > 2:305554/1‑81 (MQ=255) gAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCttt > 1:305546/1‑81 (MQ=255) aaTATCAGCCCTACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTc > 1:305561/1‑81 (MQ=255) tACAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGaa > 1:305556/1‑79 (MQ=255) cAGATCTGGATGTCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAgc < 2:305522/81‑1 (MQ=255) cAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAgc < 1:305523/81‑1 (MQ=255) tGGATGTCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAat > 1:305551/1‑81 (MQ=255) atCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAatggatg > 1:305540/1‑81 (MQ=255) | TACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATG > NC_000913/1832830‑1832988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |