| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,037,586 | 1 | . | T | 68.8% | 39.0 / 13.3 | 16 | coding (567/1359 nt) | hprS | sensory histidine kinase HprS |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base T (2/9); total (3/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAGTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAAC‑TTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGACAATGCAAAT > NC_000913/2037508‑2037663 | tAGTTCCCCCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACTACTCAAC‑tt > 2:335529/1‑81 (MQ=255) gTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAAC‑tttt < 1:335495/81‑1 (MQ=255) gTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAAC‑tttt < 2:335496/81‑1 (MQ=255) ggTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTTGAtctc < 1:335497/81‑1 (MQ=255) aTGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTTGATCTCTCg < 2:335498/80‑1 (MQ=255) aTGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAAC‑TTTTTGATCTCTCGt > 2:335527/1‑80 (MQ=255) gcTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTTGATCTCTCGTAATc < 2:335499/81‑1 (MQ=255) aCCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTTGATCTCTCGTAATCCCGt < 1:335500/81‑1 (MQ=255) gCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTTGATCTCTCGTAATCCCGTTCTGATTAAc < 1:335501/81‑1 (MQ=255) ctctCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTTGATCTCTCGTAATCCCGTTCTGATTAACa < 2:335502/81‑1 (MQ=255) ttATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATAc < 2:335503/81‑1 (MQ=255) aTTCAGCGCTTCCGTTACACCACTCAAC‑TTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCa < 1:335504/81‑1 (MQ=255) cgcTTCCGTTACACCACTCAACTTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTa < 1:335505/81‑1 (MQ=255) ccGTTACACCCCTCCAC‑TTTTTGGTCTCTCCTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATg < 1:335506/80‑1 (MQ=255) tACACCACTCAACTTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGa > 1:335531/1‑81 (MQ=255) acacCACTCAACTTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGa < 1:335507/80‑1 (MQ=255) cacTCAACTTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGACAATg > 2:335532/1‑81 (MQ=255) aaC‑TTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGACAATGCAAAt < 2:335509/80‑1 (MQ=255) aaC‑TTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGACAATGCAAAt < 1:335508/80‑1 (MQ=255) | TAGTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAAC‑TTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGACAATGCAAAT > NC_000913/2037508‑2037663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |