Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,250,2520TC66.7% 28.5 / 25.0 30F179S (TTC→TCC) yeiHconserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand):  ref base T (7/3);  new base C (6/14);  total (13/17)
Fisher's exact test for biased strand distribution p-value = 5.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCTACTGTG  >  NC_000913/2250174‑2250331
                                                                              |                                                                               
aGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAtttt                                                                                 <  1:368470/79‑1 (MQ=255)
aGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTccc                                                                               >  1:368506/1‑81 (MQ=255)
      tAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAcc                                                                         <  2:368471/81‑1 (MQ=255)
          gAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGc                                                                     >  1:368508/1‑81 (MQ=255)
           aaaGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGc                                                                     <  2:368472/80‑1 (MQ=255)
           aaaGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCg                                                                    <  1:368473/81‑1 (MQ=255)
              gCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGata                                                                 <  2:368474/81‑1 (MQ=255)
                     ccAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGc                                                          <  1:368475/81‑1 (MQ=255)
                       agtaaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCt                                                         >  1:368509/1‑80 (MQ=255)
                         taaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGAt                                                      >  2:368520/1‑81 (MQ=255)
                             gtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct                                                  >  2:368524/1‑81 (MQ=255)
                             gtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGtct                                                  <  2:368476/81‑1 (MQ=255)
                              taaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGtctc                                                 <  2:368477/81‑1 (MQ=255)
                               aaacGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGtctc                                                 >  2:368516/4‑80 (MQ=255)
                                    tGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGt                                           >  1:368513/1‑81 (MQ=255)
                                     ggCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGtt                                          >  1:368512/1‑81 (MQ=255)
                                     ggCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGtt                                          >  2:368517/1‑81 (MQ=255)
                                      gCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt                                         <  1:368478/81‑1 (MQ=255)
                                          ttGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAg                                       <  1:368479/79‑1 (MQ=255)
                                            gCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCg                                   >  2:368532/1‑81 (MQ=255)
                                               aCCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaa                                <  2:368480/81‑1 (MQ=255)
                                                   ttgttATCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACtt                            <  1:368481/81‑1 (MQ=255)
                                                         tCTTCGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGt                       <  1:368482/80‑1 (MQ=255)
                                                            tCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTAATTCCGGAACCTTTCGGTacc                    >  2:368515/1‑78 (MQ=255)
                                                             cGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTAt                     <  1:368484/78‑1 (MQ=255)
                                                             cGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTAt                     <  1:368485/78‑1 (MQ=255)
                                                             cGGGACCGTCGCGATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTAt                     <  2:368483/78‑1 (MQ=255)
                                                                    gTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGt           >  2:368521/1‑81 (MQ=255)
                                                                       gcgATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCt        <  2:368486/81‑1 (MQ=255)
                                                                         gATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCTAc      >  2:368531/1‑81 (MQ=255)
                                                                         gATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCTAc      <  2:368487/81‑1 (MQ=255)
                                                                         gATTTCCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCTAc      >  1:368514/1‑81 (MQ=255)
                                                                             ttCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCTACtgtg  >  2:368540/1‑81 (MQ=255)
                                                                             ttCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCTACtgt   <  2:368488/80‑1 (MQ=255)
                                                                              |                                                                               
AGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTTCGGTATCTACATTGGTTCTACTGTG  >  NC_000913/2250174‑2250331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑