Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,341,5040TC67.7% 34.4 / 27.1 31F80S (TTC→TCC) kdsDD‑arabinose 5‑phosphate isomerase KdsD
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base C (8/13);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCT  >  NC_000913/3341428‑3341583
                                                                            |                                                                               
aTGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGt                                                                              <  2:580058/80‑1 (MQ=255)
 tGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTcc                                                                            <  1:580059/81‑1 (MQ=255)
      aTGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCt                                                                       <  2:580060/81‑1 (MQ=255)
      aTGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCt                                                                       <  2:580061/81‑1 (MQ=255)
      aTGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCt                                                                       >  1:580109/1‑81 (MQ=255)
       tGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTg                                                                      <  1:580062/81‑1 (MQ=255)
         ggAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGt                                                                    <  2:580063/81‑1 (MQ=255)
          gAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTg                                                                   <  1:580064/81‑1 (MQ=255)
           aaaaTCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTg                                                                   >  1:580104/1‑80 (MQ=255)
           aaaaTCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTg                                                                   >  2:580112/1‑80 (MQ=255)
            aaaTCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGaa                                                                 <  1:580065/81‑1 (MQ=255)
                     cATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCAt                                                        <  2:580066/81‑1 (MQ=255)
                     cATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCAt                                                        >  2:580108/1‑81 (MQ=255)
                           gggCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGAt                                                  >  2:580105/1‑81 (MQ=255)
                           gggCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGa                                                   <  2:580067/80‑1 (MQ=255)
                           gggCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGAt                                                  >  1:580107/1‑81 (MQ=255)
                             gCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGAttt                                                <  2:580068/81‑1 (MQ=255)
                                       gcggcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGt                                       >  1:580121/1‑80 (MQ=255)
                                       gcggcAACGTTTGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGt                                       >  1:580117/1‑80 (MQ=255)
                                               gTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCca                                >  1:580115/1‑79 (MQ=255)
                                                tttGCCAGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGTGCATGGTGATTTAGGCATGGTTACCCCACAg                             >  2:580131/1‑81 (MQ=255)
                                                      aGCACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGAtgtg                       >  1:580124/1‑81 (MQ=255)
                                                        cACCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATgtggt                     <  1:580069/81‑1 (MQ=255)
                                                         aCCGGTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATgtggt                     >  1:580113/1‑80 (MQ=255)
                                                            ggTACACCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGAtt                 <  2:580070/81‑1 (MQ=255)
                                                                cacCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCt              <  1:580072/80‑1 (MQ=255)
                                                                cacCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCt              <  1:580073/80‑1 (MQ=255)
                                                                cacCTTCATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCt              <  1:580071/80‑1 (MQ=255)
                                                                   cTTCATTTTCCGTCCATCCTGGTGATGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTAtct          <  1:580074/81‑1 (MQ=255)
                                                                      cATTTTCCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATctct        >  2:580136/1‑80 (MQ=255)
                                                                       aTTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTaa      <  2:580075/81‑1 (MQ=255)
                                                                          tttCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAAct    <  1:580077/80‑1 (MQ=255)
                                                                            tCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAActct  <  2:580078/80‑1 (MQ=255)
                                                                            |                                                                               
ATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCT  >  NC_000913/3341428‑3341583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑