Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,122	A→C	I220S (ATC→AGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,122	0	A	C	100.0%	124.1 / NA	34	I220S (ATC→AGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/14);  total (20/14)

ACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTC  >  NC_000913/65046‑65195                                                                             |                                                                          aCGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTcac                                                                       <  2:14008/81‑1 (MQ=255)    cAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATc                                                                    <  2:14009/81‑1 (MQ=255)       cGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAgg                                                                 <  2:14010/81‑1 (MQ=255)        ggAAGACGGTAACGCTCGGCATGTTTTTGCAGCATCCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGa                                                                >  1:14062/1‑81 (MQ=255)          aaGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATc                                                              >  2:14047/1‑81 (MQ=255)               ggTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGt                                                         <  1:14011/81‑1 (MQ=255)               ggTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGt                                                         >  2:14055/1‑81 (MQ=255)                     gCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCa                                                    >  1:14068/1‑80 (MQ=255)                        cGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAAc                                                 >  2:14052/1‑80 (MQ=255)                        cGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAAc                                                 >  1:14049/1‑80 (MQ=255)                               tttttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGtt                                         <  2:14013/81‑1 (MQ=255)                               tttttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGtt                                         <  2:14012/81‑1 (MQ=255)                                  ttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGa                                       <  2:14014/80‑1 (MQ=255)                                    gcagcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGtt                                    >  1:14050/1‑81 (MQ=255)                                    gcagcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGt                                     <  2:14015/80‑1 (MQ=255)                                      agcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGtttt                                  <  1:14016/81‑1 (MQ=255)                                        caTTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTc                                >  2:14074/1‑81 (MQ=255)                                        caTTCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTc                                >  1:14051/1‑81 (MQ=255)                                          ttCGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTc                                >  1:14048/1‑79 (MQ=255)                                            cGCAGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAg                             >  2:14072/1‑80 (MQ=255)                                               aGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAAc                         >  1:14059/1‑81 (MQ=255)                                               aGATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAAc                         >  1:14075/1‑81 (MQ=255)                                                gATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACt                        >  2:14056/1‑81 (MQ=255)                                                gATCGAACTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACt                        >  2:14046/1‑81 (MQ=255)                                                      aCTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTgcgggc                  <  1:14017/81‑1 (MQ=255)                                                       cTGCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTgcgggcg                 >  1:14057/1‑81 (MQ=255)                                                         gCACCACGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGggcggc               >  1:14070/1‑81 (MQ=255)                                                          caccacGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGggcggc               <  2:14018/80‑1 (MQ=255)                                                          caccacGTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCt              <  2:14019/81‑1 (MQ=255)                                                                gTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGAc        <  1:14020/81‑1 (MQ=255)                                                                gTTCCAACCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGAc        <  2:14021/81‑1 (MQ=255)                                                                     aaCCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTAtt   >  1:14071/1‑81 (MQ=255)                                                                      aCCGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTc  >  1:14054/1‑81 (MQ=255)                                                                       ccGATGCTCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTc  >  1:14067/1‑80 (MQ=255)                                                                             |                                                                          ACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTC  >  NC_000913/65046‑65195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑