Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,192	T→A	S1023T (TCC→ACC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,192	0	T	A	100.0%	91.3 / NA	25	S1023T (TCC→ACC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/11);  total (14/11)

GCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACT  >  NC_000913/208114‑208268                                                                               |                                                                             gCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTAcc                                                                            >  2:44870/1‑81 (MQ=255)   ggggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCgg                                                                          >  2:44863/1‑81 (MQ=255)      gCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGggcg                                                                       <  1:44830/81‑1 (MQ=255)         cGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                                    >  2:44866/1‑81 (MQ=255)          gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                                    <  1:44831/80‑1 (MQ=255)          gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                                    <  2:44832/80‑1 (MQ=255)          gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTg                                                                   >  1:44860/1‑81 (MQ=255)           ttgttgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTg                                                                   >  2:44872/1‑80 (MQ=255)             gttgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGa                                                                 <  2:44833/80‑1 (MQ=255)                   gcgcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtg                                                          >  1:44874/1‑81 (MQ=255)                     gcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgtt                                                        <  2:44834/81‑1 (MQ=255)                       gggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgttgt                                                      >  1:44871/1‑81 (MQ=255)                        ggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGAACGCTGGATGAACGTACCGGGCGGCTGGAAGTGATgttgtt                                                     <  2:44835/81‑1 (MQ=255)                               gTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGa                                               <  2:44836/80‑1 (MQ=255)                                 cACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACga                                            >  2:44853/1‑80 (MQ=255)                                              aaTCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATAc                               >  2:44867/1‑81 (MQ=255)                                               aTCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATAc                               >  2:44864/1‑80 (MQ=255)                                                      cGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAAtt                       >  1:44865/1‑81 (MQ=255)                                                       ggTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAAtt                       <  2:44837/80‑1 (MQ=255)                                                            cTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTgg                  >  1:44868/1‑80 (MQ=255)                                                               cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTgg                  <  2:44838/77‑1 (MQ=255)                                                               cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGa                 <  2:44839/78‑1 (MQ=255)                                                                     ggATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCg        >  2:44877/1‑81 (MQ=255)                                                                        tGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCAt     <  2:44840/81‑1 (MQ=255)                                                                           ccGTACCGGGCGGCTGGAAGTGATGTTGATTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACt  >  2:44873/1‑81 (MQ=255)                                                                               |                                                                             GCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACT  >  NC_000913/208114‑208268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑