Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,075,218	C→A	A147E (GCG→GAG)	yeeR →	CP4‑44 prophage; inner membrane protein YeeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,075,218	0	C	A	100.0%	88.5 / NA	24	A147E (GCG→GAG)	yeeR	CP4‑44 prophage; inner membrane protein YeeR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/13);  total (11/13)

CGGGATCACACCAGAAGATATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGCGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGT  >  NC_000913/2075141‑2075295                                                                              |                                                                              cGGGATCACACCAGAAGATATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAgtg                                                                            >  1:340903/1‑81 (MQ=255)  gggATCACACCAGAAGATATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAgtgt                                                                           >  2:340909/1‑81 (MQ=255)   ggATCACACCAGAAGATATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGtt                                                                          <  2:340878/81‑1 (MQ=255)     aTCACACCAGAAGATATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCt                                                                        >  1:340914/1‑81 (MQ=255)      tCACACCAGAAGATATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTg                                                                       >  2:340907/1‑81 (MQ=255)             agaagaTATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATgcgc                                                                <  2:340879/81‑1 (MQ=255)             agaagaTATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCAGCATgcgc                                                                <  1:340880/81‑1 (MQ=255)                   tatCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCAtt                                                          <  2:340881/81‑1 (MQ=255)                       aGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATc                                                      <  2:340882/81‑1 (MQ=255)                        gCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATcg                                                     >  1:340910/1‑81 (MQ=255)                         cAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATcgc                                                    <  1:340883/81‑1 (MQ=255)                         cAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATcgc                                                    <  1:340884/81‑1 (MQ=255)                            ggAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGtt                                                 <  1:340885/81‑1 (MQ=255)                                 ttAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGt                                            <  1:340886/81‑1 (MQ=255)                                   aaCGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTa                                           <  1:340887/80‑1 (MQ=255)                                           ttACTCCTGTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCCCACCATTGATCCCGTTACAGTACGATATTTc                                  >  1:889331/1‑81 (MQ=255)                                                 ctgTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCg                              <  2:340888/79‑1 (MQ=255)                                                   gTTTTTATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCAc                          >  1:340904/1‑81 (MQ=255)                                                       ttATACTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGa                       <  1:340889/80‑1 (MQ=255)                                                        tataCTGCCTTTTTCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGAtg                     >  2:340908/1‑81 (MQ=255)                                                                  ttttCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGATGTGGTGGGGCg           >  1:340911/1‑81 (MQ=255)                                                                   tttCCTTATGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGATGTGGTGGGGCgg          >  1:340915/1‑81 (MQ=255)                                                                          aTGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGATGTGGTGGGGCGGGttgttg   >  1:340906/1‑81 (MQ=255)                                                                           tGGAGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGATGTGGTGGGGCGGGTtgttgt  <  2:340890/81‑1 (MQ=255)                                                                              |                                                                              CGGGATCACACCAGAAGATATCAGCAAGGAAATTAACGGGTATTACTGCTGTTTTTATACTGCCTTTTTCCTTATGGCGTGTTCTGCATGCGCACCATTGATCGCGTTACAGTACGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGT  >  NC_000913/2075141‑2075295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑