Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,517	A→T	D271E (GAT→GAA)	glf ←	UDP‑galactopyranose mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,517	0	A	T	100.0%	41.7 / NA	12	D271E (GAT→GAA)	glf	UDP‑galactopyranose mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/4);  total (8/4)

CGTATGCTTTGTCTCAACATAGTCAAAATGTTTATGCTCAATTATTCTGGTATATGGTACATTAGCATCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTCCGTCTCAAATTTTA  >  NC_000913/2107451‑2107584                                                                   |                                                                    cGTATGCTTTGTCTCAACATAGTCAAAATGTTTATGCTCAATTATTCTGGTATATGGTACATTAGCTTCAGTGAAATttat                                                       <  2:345459/81‑1 (MQ=255)                       tCAAAATGTTTATGCTCAATTATTCTGGTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGttt                                 <  1:345460/81‑1 (MQ=255)                              gTTTATGCTCAATTATTCTGGTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAAtt                          >  2:345483/1‑81 (MQ=255)                               tttATGCTCAATTATTCTGGTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAAtt                          >  1:345478/1‑80 (MQ=255)                                     cTCAATTATTCTGGTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCg                   >  2:345481/1‑81 (MQ=255)                                        aaTTATTCTGGTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGtt                 <  1:345461/80‑1 (MQ=255)                                         attattCTGGTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTcc               <  2:345462/81‑1 (MQ=255)                                           tattCTGGTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTCCGt             >  2:345482/1‑81 (MQ=255)                                                 ggTATATGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTCCGTCTCaaa       >  1:345485/1‑81 (MQ=255)                                                    atatGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTCCGTCTCAAAttt    >  1:345476/1‑81 (MQ=255)                                                       tGGTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTCCGTCTCAAATTTTa  >  2:345477/1‑80 (MQ=255)                                                         gTACATTAGCTTCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTCCGTCTCAAATTTTa  >  1:345500/1‑78 (MQ=255)                                                                   |                                                                    CGTATGCTTTGTCTCAACATAGTCAAAATGTTTATGCTCAATTATTCTGGTATATGGTACATTAGCATCAGTGAAATTTATTACTGCATTCCCTTGGAAGTTTGGAAATTCATGGCGTTCCGTCTCAAATTTTA  >  NC_000913/2107451‑2107584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑