Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,140,712	C→A	E301* (GAA→TAA)	asmA ←	putative assembly protein AsmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,140,712	0	C	A	100.0%	69.1 / NA	19	E301* (GAA→TAA)	asmA	putative assembly protein AsmA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/7);  total (12/7)

GAAATGACCGGGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCT  >  NC_000913/2140639‑2140780                                                                          |                                                                     gaaATGACCGGGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGc                                                               <  2:350469/81‑1 (MQ=255)          gggCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAgg                                                      <  1:350470/81‑1 (MQ=255)          gggCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAgg                                                      >  2:350495/1‑81 (MQ=255)             cgcgGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTg                                                   >  2:350500/1‑81 (MQ=255)                ggCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTc                                                 >  1:350504/1‑80 (MQ=255)                   aaCGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTc                                                 >  2:350492/1‑77 (MQ=255)                    aCGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGAt                                            <  1:350471/81‑1 (MQ=255)                      gTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGAtt                                           >  2:350499/1‑80 (MQ=255)                             ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGc                                    <  1:350472/80‑1 (MQ=255)                                    ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTg                            >  2:350505/1‑81 (MQ=255)                                    ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTg                            >  1:350502/1‑81 (MQ=255)                                      ccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCa                          <  1:350473/81‑1 (MQ=255)                                       ctgctgCGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAg                         >  1:350507/1‑81 (MQ=255)                                           tgcGCGGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCt                      >  1:350506/1‑80 (MQ=255)                                              gcgGCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGc                  >  1:350511/1‑81 (MQ=255)                                                ggCACCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTg                >  2:350510/1‑81 (MQ=255)                                                   aCCGTTTTCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCa             <  2:350474/81‑1 (MQ=255)                                                       ttttCACCATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTcc         <  1:350475/81‑1 (MQ=255)                                                              cATTCGCGGTTTAATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTtct  >  2:350509/1‑81 (MQ=255)                                                                          |                                                                     GAAATGACCGGGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCT  >  NC_000913/2140639‑2140780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑