| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,183,270 | C→A | A128S (GCG→TCG) | yegX ← | putative hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,183,270 | 0 | C | A | 80.0% | 38.4 / 8.3 | 20 | A128S (GCG→TCG) | yegX | putative hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base A (8/8); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GTGAGAAATCCACCGTTTGCAGAAATAATCTCGCCTGAACTGAAGCGGATACCGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTC > NC_000913/2183195‑2183349 | gtgAGAAATCCACCGTTTGCAGAAATAATCTCGCCTGAACTGAAGCGGATACCGACGGGGAGAAATAATGATACGACCCa < 1:357613/80‑1 (MQ=255) tgAGAAATCCACCGTTTGCAGAAATAATCTCGCCTGAACTGAAGCGGATACCGACGGGGAGAAATAATGATACGACCCAc < 1:357614/80‑1 (MQ=255) cACCGTTTGCAGAAATAATCTCGCCTGAACTGAAGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGc > 1:357634/1‑80 (MQ=255) aatCTCGCCTGAACTGAAGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGt > 2:357626/1‑81 (MQ=255) cGCCTGAACTGAAGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGc > 2:357639/1‑79 (MQ=255) aactgaaGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTAcg < 1:357615/81‑1 (MQ=255) aactgaaGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTAc < 2:357616/80‑1 (MQ=255) ctgaaGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTAcgc > 1:357640/1‑80 (MQ=255) aaGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGaa > 1:357647/1‑80 (MQ=255) aGCGGATACCGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGaaa > 1:357649/1‑80 (MQ=255) ccGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAgggg < 1:357617/80‑1 (MQ=255) cGACGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTc < 1:357618/81‑1 (MQ=255) aCGGGGAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTcca < 1:357619/81‑1 (MQ=255) gggAGAAATAATGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTccacca < 1:357620/81‑1 (MQ=255) gaAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCtt > 1:357660/1‑81 (MQ=255) gaAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCtt > 2:357646/1‑81 (MQ=255) aaaTAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCttt > 2:357661/1‑81 (MQ=255) aataatGATACGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCtttt > 2:357648/1‑81 (MQ=255) aCGACCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATCGGGGTCCACCAGCTTTTCGCCTTCCg > 1:357669/1‑81 (MQ=255) gCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTc > 2:357654/1‑81 (MQ=255) | GTGAGAAATCCACCGTTTGCAGAAATAATCTCGCCTGAACTGAAGCGGATACCGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTC > NC_000913/2183195‑2183349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |