Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,249,633	T→C	intergenic (‑15/‑84)	yeiE ← / → yeiH	putative LysR‑type transcriptional regulator YeiE/conserved inner membrane protein YeiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,249,633	0	T	C	100.0%	27.9 / NA	9	intergenic (‑15/‑84)	yeiE/yeiH	putative LysR‑type transcriptional regulator YeiE/conserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/7);  total (2/7)

GGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGT  >  NC_000913/2249556‑2249687                                                                              |                                                       ggtggtTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTcat                                                      <  2:368402/80‑1 (MQ=255)     gtTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTCATAAAGa                                                 <  1:368403/81‑1 (MQ=255)      ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACAGCCGGAGGGTGATGTGCATAGTCGCTTACCACTCATAAAGat                                                <  1:368404/81‑1 (MQ=255)         aTCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTCATAAAGatta                                              <  2:368405/80‑1 (MQ=255)          tCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTCATAAAGattaat                                            <  1:368406/81‑1 (MQ=255)                    aaTACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTCATAAAGATTAATTATAAatata                                  >  1:368415/1‑81 (MQ=255)                     aTACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTCATAAAGATTAATTATAAatata                                  >  2:368414/1‑80 (MQ=255)                           cTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTCATAAAGATTAATTATAAATATATAATCa                            <  1:368407/80‑1 (MQ=255)                                                  aGGGTGATGTGCATAGTCGCTTACCACTCATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTc    <  1:368408/81‑1 (MQ=255)                                                    ggTGATGTGCATAGTCGCTTACCACTCATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGt  <  1:368409/81‑1 (MQ=255)                                                                              |                                                       GGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGT  >  NC_000913/2249556‑2249687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑