Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,134	T→A	N55Y (AAC→TAC)	psuK ←	putative pseudouridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,134	0	T	A	100.0%	71.0 / NA	20	N55Y (AAC→TAC)	psuK	putative pseudouridine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/11);  total (9/11)

CCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATT  >  NC_000913/2259058‑2259206                                                                             |                                                                         ccAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAAccc                                                                      <  2:369866/81‑1 (MQ=255) ccAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAAccc                                                                      <  1:369867/81‑1 (MQ=255)  cAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAAcc                                                                       >  2:369898/1‑79 (MQ=255)      ttgattgGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCaa                                                                 >  2:369894/1‑81 (MQ=255)        gattgGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAAc                                                                <  1:369868/80‑1 (MQ=255)          ttgGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGc                                                              >  1:369874/1‑80 (MQ=255)                   gTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTtgt                                                    >  2:369889/1‑81 (MQ=255)                                   cATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTa                                    >  2:369897/1‑81 (MQ=255)                                     tAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACt                                  <  2:369869/81‑1 (MQ=255)                                           tCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTccacca                            <  2:369870/81‑1 (MQ=255)                                             aCTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACTCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAgg                          >  2:369891/1‑81 (MQ=255)                                              cTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGc                         <  2:369871/81‑1 (MQ=255)                                                  cTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTaa                     <  2:369872/81‑1 (MQ=255)                                                     cgacgcTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCTCCAGGCGTaaa                    <  2:369873/76‑1 (MQ=255)                                                        cgcTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAatttta               <  2:369874/81‑1 (MQ=255)                                                         gcTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAattttat              >  2:369899/1‑81 (MQ=255)                                                            cAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGTGCCCTACTCCACCAGGCGTAAattttattt            >  2:369896/1‑80 (MQ=255)                                                               tAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTAcc        <  2:369875/81‑1 (MQ=255)                                                                    aGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGAt   >  1:369902/1‑81 (MQ=255)                                                                     ggCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGAtt  <  1:369876/81‑1 (MQ=255)                                                                             |                                                                         CCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATT  >  NC_000913/2259058‑2259206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑