Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,275,772	A→G	Y167C (TAT→TGT)	yejE →	putative oligopeptide ABC transporter membrane subunit YejE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,275,772	0	A	G	100.0%	119.9 / NA	33	Y167C (TAT→TGT)	yejE	putative oligopeptide ABC transporter membrane subunit YejE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

GTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATT  >  NC_000913/2275702‑2275848                                                                       |                                                                             gTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGt                                                                    <  2:372543/81‑1 (MQ=255)  ttCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTa                                                                   <  1:372544/81‑1 (MQ=255)   tCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTa                                                                   >  1:372583/1‑80 (MQ=255)   tCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTa                                                                   >  1:372597/1‑80 (MQ=255)         tGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCg                                                             <  1:372545/80‑1 (MQ=255)         tGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCg                                                             <  2:372546/80‑1 (MQ=255)          ggCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGAt                                                           >  1:372582/1‑81 (MQ=255)           gCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGAtc                                                          <  2:372547/81‑1 (MQ=255)             cTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGAtctc                                                        <  2:372548/81‑1 (MQ=255)                            tGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCt                                           <  2:372549/79‑1 (MQ=255)                              ttCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCttt                                         <  1:372550/79‑1 (MQ=255)                              ttCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTa                                        <  1:372551/80‑1 (MQ=255)                                    cGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGTTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGt                                 <  2:372552/81‑1 (MQ=255)                                       gATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATg                              >  2:372606/1‑81 (MQ=255)                                       gATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATg                              >  2:372598/1‑81 (MQ=255)                                       gATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATg                              >  1:372599/1‑81 (MQ=255)                                       gATGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATg                              >  1:372590/1‑81 (MQ=255)                                        aTGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATgg                             >  1:372589/1‑81 (MQ=255)                                        aTGGGCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATg                              <  1:372553/80‑1 (MQ=255)                                           ggCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCg                          >  1:372592/1‑81 (MQ=255)                                           ggCGTGCTGGCGGGGGCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCg                          <  1:372554/81‑1 (MQ=255)                                                       ggggCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGATGTATGGTCGGGGATGCCGACg              >  1:372591/1‑81 (MQ=255)                                                        gggCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGTTTTATTGAAGTATGGTCGGGGATGCCGACGc             >  2:372588/1‑81 (MQ=255)                                                         ggCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCt            >  1:372609/1‑81 (MQ=255)                                                         ggCGCTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCt            >  2:372603/1‑81 (MQ=255)                                                          gcgcTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTg           <  2:372555/81‑1 (MQ=255)                                                            gcTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGtt         <  1:372556/81‑1 (MQ=255)                                                             cTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGttt        <  2:372557/81‑1 (MQ=255)                                                             cTACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGttt        <  2:372558/81‑1 (MQ=255)                                                              tACAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGtttt       >  1:372605/1‑81 (MQ=255)                                                                cAAGGCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGtttt       <  1:372559/79‑1 (MQ=255)                                                                   ggCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGatt  <  1:372560/81‑1 (MQ=255)                                                                   ggCTGTTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGat   >  1:372604/1‑80 (MQ=255)                                                                       |                                                                             GTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATT  >  NC_000913/2275702‑2275848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑