Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,451,016	A→G	D20D (GAT→GAC)	yfcQ ←	putative fimbrial protein YfcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,451,016	0	A	G	100.0%	78.2 / NA	22	D20D (GAT→GAC)	yfcQ	putative fimbrial protein YfcQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

ACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCAC  >  NC_000913/2450942‑2451089                                                                           |                                                                          aCTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcagca                                                                     <  2:403836/81‑1 (MQ=255)   ttCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcagcag                                                                    >  2:403868/1‑80 (MQ=255)     cAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCAt                                                                 >  1:403878/1‑81 (MQ=255)         ggTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGc                                                             >  1:403883/1‑81 (MQ=255)              tACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATg                                                         <  2:403837/80‑1 (MQ=255)               accaccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATgg                                                        <  1:403838/80‑1 (MQ=255)               accaccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgc                                                       >  1:403876/1‑81 (MQ=255)                  accGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgcgc                                                     >  2:403874/1‑80 (MQ=255)                      cTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTg                                                <  1:403839/81‑1 (MQ=255)                          tGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGacac                                            <  1:403840/81‑1 (MQ=255)                            cTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGAcaca                                           <  1:403841/80‑1 (MQ=255)                              aCAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAg                                        >  2:403872/1‑81 (MQ=255)                               cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACaaa                                         <  2:403842/79‑1 (MQ=255)                               cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGc                                       <  1:403843/81‑1 (MQ=255)                                         gTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGa                             <  1:403844/81‑1 (MQ=255)                                                     tCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTtctc                  <  1:403845/80‑1 (MQ=255)                                                            aaaGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAg          >  1:403882/1‑81 (MQ=255)                                                             aaGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGt         >  1:403879/1‑81 (MQ=255)                                                               ggTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTcc       <  1:403847/81‑1 (MQ=255)                                                               ggTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTcc       <  1:403846/81‑1 (MQ=255)                                                                   aTGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTccacc    <  1:403848/80‑1 (MQ=255)                                                                     gTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTccaccac  >  2:403881/1‑80 (MQ=255)                                                                           |                                                                          ACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCAC  >  NC_000913/2450942‑2451089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑