Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,513,154	T→C	V38A (GTT→GCT)	nupC →	nucleoside:H(+) symporter NupC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,513,154	0	T	C	100.0%	120.8 / NA	33	V38A (GTT→GCT)	nupC	nucleoside:H(+) symporter NupC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

TTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGTTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTCCGAAATG  >  NC_000913/2513079‑2513230                                                                            |                                                                             ttgttgCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATc                                                                          >  1:413499/1‑80 (MQ=255) ttgttgCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCg                                                                         <  2:413456/81‑1 (MQ=255)    ttgCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAg                                                                      <  1:413457/81‑1 (MQ=255)        gATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGtt                                                                  <  1:413458/81‑1 (MQ=255)          ttCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTAc                                                                <  2:413459/81‑1 (MQ=255)                 aCTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTg                                                         >  2:413494/1‑81 (MQ=255)                     ctgGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGtt                                                      <  2:413460/80‑1 (MQ=255)                             cagcGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGaa                                             >  1:413496/1‑81 (MQ=255)                              agcGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAAc                                            >  2:413492/1‑81 (MQ=255)                                cGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTc                                          >  2:413504/1‑81 (MQ=255)                                 gACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTcc                                         <  1:413461/81‑1 (MQ=255)                                 gACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTcc                                         <  2:413462/81‑1 (MQ=255)                                    cGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGAc                                      <  2:413463/81‑1 (MQ=255)                                      cAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGt                                    <  2:413464/81‑1 (MQ=255)                                       aagaaaaTTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGtt                                   <  2:413465/78‑1 (MQ=255)                                         aaaaaTTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCTTGAACTCCGACGTTgg                                 >  1:413501/1‑81 (MQ=255)                                           aaaTTCGTATCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGtt                               <  2:413466/81‑1 (MQ=255)                                                    tCCGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCg                      >  1:413500/1‑81 (MQ=255)                                                     ccGTTATGTTATTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGt                     >  2:413502/1‑81 (MQ=255)                                                       gttatgttatTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTg                    >  2:413505/1‑80 (MQ=255)                                                         tatgttatTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGa                   >  1:413493/1‑79 (MQ=255)                                                            gttatTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGc              >  1:413506/1‑81 (MQ=255)                                                              tatTCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCtt            >  1:413507/1‑81 (MQ=255)                                                                ttCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTtct          <  1:413468/81‑1 (MQ=255)                                                                ttCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTtct          <  2:413469/81‑1 (MQ=255)                                                                ttCAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTtct          <  2:413467/81‑1 (MQ=255)                                                                  cAACTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTtctc         <  1:413470/80‑1 (MQ=255)                                                                   aaCTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTcc        <  2:413471/80‑1 (MQ=255)                                                                   aaCTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTcc        <  1:413473/80‑1 (MQ=255)                                                                   aaCTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTCCg       <  2:413472/81‑1 (MQ=255)                                                                     cTGCTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTCCGa      >  2:413503/1‑80 (MQ=255)                                                                        cTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTCCGAAAt   <  2:413474/80‑1 (MQ=255)                                                                        cTTGCTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTCCGAAATg  <  1:413475/81‑1 (MQ=255)                                                                            |                                                                             TTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGTTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTTTAGGCTTCGTGAAAGGCTTCTCCGAAATG  >  NC_000913/2513079‑2513230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑