Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,660,768	G→C	A255G (GCC→GGC)	iscS ←	cysteine desulfurase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,660,768	0	G	C	100.0%	119.7 / NA	33	A255G (GCC→GGC)	iscS	cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/21);  total (12/21)

CACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCAT  >  NC_000913/2660699‑2660844                                                                      |                                                                             cacaGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCAtt                                                                   <  1:441394/81‑1 (MQ=255)   caGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTcc                                                                 <  1:441395/81‑1 (MQ=255)       cGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCcgacg                                                             <  2:441397/81‑1 (MQ=255)       cGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCcgacg                                                             <  2:441396/81‑1 (MQ=255)            aCGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTg                                                        >  1:441470/1‑81 (MQ=255)             cGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTg                                                        <  2:441398/80‑1 (MQ=255)                aGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTg                                                     >  2:441459/1‑80 (MQ=255)                 ggCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGaa                                                   >  2:441447/1‑81 (MQ=255)                  gCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAAc                                                  <  2:441399/81‑1 (MQ=255)                   ccGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACa                                                 <  2:441400/81‑1 (MQ=255)                       gcAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCa                                             <  2:441401/81‑1 (MQ=255)                       gcAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCa                                             <  1:441402/81‑1 (MQ=255)                           aCGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGt                                         <  1:441403/81‑1 (MQ=255)                            cGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTg                                        <  1:441404/81‑1 (MQ=255)                            cGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTg                                        >  1:441451/1‑81 (MQ=255)                              ttCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGcc                                      >  2:441464/1‑81 (MQ=255)                                 cATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCgg                                    >  1:441406/1‑80 (MQ=255)                                 cATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCgg                                    >  2:441452/1‑80 (MQ=255)                                 cATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGa                                   <  2:441405/81‑1 (MQ=255)                                       ggTCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCAt                             <  2:441407/81‑1 (MQ=255)                                         tCGCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATAc                           >  2:441460/1‑81 (MQ=255)                                           gCCATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATAcc                          <  1:441408/80‑1 (MQ=255)                                            ccATCTCTTCTTTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACcgc                        <  2:441409/81‑1 (MQ=255)                                                     cttTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGt                  <  1:441410/78‑1 (MQ=255)                                                     cttTTGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGa                <  1:441411/80‑1 (MQ=255)                                                       tttGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccg             >  1:441486/1‑81 (MQ=255)                                                        ttGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccg             <  1:441413/80‑1 (MQ=255)                                                        ttGCGATGCGATAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAc               <  2:441412/78‑1 (MQ=255)                                                           cgatgcgatAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgccgc         <  2:441414/81‑1 (MQ=255)                                                           cgatgcgatAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgccgc         >  2:441461/1‑81 (MQ=255)                                                             atgcgatAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgccgccg       >  1:441467/1‑81 (MQ=255)                                                                cgatAGCCCTCGCCCATTCCGACGATCTGGTAAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGc    <  2:441415/81‑1 (MQ=255)                                                                  atAGCCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCAt  >  1:441463/1‑81 (MQ=255)                                                                      |                                                                             CACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCAT  >  NC_000913/2660699‑2660844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑