Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,692,082	C→G	W1154C (TGG→TGC)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,692,082	0	C	G	100.0%	167.5 / NA	46	W1154C (TGG→TGC)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (17/29);  total (17/29)

GACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGCCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCCCAGCGCCAGCGT  >  NC_000913/2692008‑2692159                                                                           |                                                                              gaCGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAAc                                                                         <  1:447591/81‑1 (MQ=255)     ggCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCAc                                                                     <  1:447592/81‑1 (MQ=255)      gCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACt                                                                    <  1:447594/81‑1 (MQ=255)      gCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACt                                                                    <  2:447593/81‑1 (MQ=255)         ttGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTAcc                                                                 >  2:447647/1‑81 (MQ=255)         ttGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTAcc                                                                 >  1:447644/1‑81 (MQ=255)          tGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTAcc                                                                 <  2:447595/80‑1 (MQ=255)            ggTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTgg                                                              <  1:447596/81‑1 (MQ=255)             gTTACTTCAACCAGGCTGAAACGCGCTTCAAGGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTggg                                                             <  2:447597/81‑1 (MQ=255)               tACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGAt                                                           >  2:447649/1‑81 (MQ=255)                aCTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGAt                                                           <  1:447598/80‑1 (MQ=255)                     aaCCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGtt                                                      <  1:447599/80‑1 (MQ=255)                      aCCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCa                                                    <  2:447600/81‑1 (MQ=255)                         aGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGc                                                 <  2:447602/81‑1 (MQ=255)                         aGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGc                                                 <  1:447601/81‑1 (MQ=255)                            cTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACg                                                  >  1:447655/1‑77 (MQ=255)                            cTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACg                                                  >  1:447667/1‑77 (MQ=255)                             tGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGAt                                             >  1:447652/1‑81 (MQ=255)                              gAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGAt                                             >  2:447642/1‑80 (MQ=255)                               aaaCGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGAt                                             >  1:447637/1‑79 (MQ=255)                               aaaCGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGAt                                             <  2:447603/79‑1 (MQ=255)                               aaaCGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTa                                           <  1:447604/81‑1 (MQ=255)                                aaCGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAg                                          <  2:447605/81‑1 (MQ=255)                                       ttCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGAcatcat                                   >  1:447660/1‑81 (MQ=255)                                       ttCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGAcatcat                                   >  2:447645/1‑81 (MQ=255)                                          aaaGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCt                                 <  2:447607/80‑1 (MQ=255)                                          aaaGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCt                                 <  1:447606/80‑1 (MQ=255)                                           aaGCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTgg                               >  2:447663/1‑81 (MQ=255)                                            aGCGATCGGGGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGc                              <  2:447608/81‑1 (MQ=255)                                             gCGATCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGc                              >  1:447646/1‑80 (MQ=255)                                                aTCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAAcc                          <  2:447609/81‑1 (MQ=255)                                                aTCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAAcc                          <  2:447610/81‑1 (MQ=255)                                                aTCGGAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAAcc                          <  2:447611/81‑1 (MQ=255)                                                   ggAGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGt                        <  2:447612/80‑1 (MQ=255)                                                     aGGTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTAc                     <  2:447613/81‑1 (MQ=255)                                                       gTATTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACAt                   <  2:447614/81‑1 (MQ=255)                                                         aTTGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATAc                 >  1:447665/1‑81 (MQ=255)                                                          ttGCGCACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATAcc                <  1:447615/81‑1 (MQ=255)                                                             cgcACAAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATAcccc              <  1:447616/80‑1 (MQ=255)                                                               cacaAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCccag           >  2:447656/1‑81 (MQ=255)                                                                acaAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCccagc          <  2:447617/81‑1 (MQ=255)                                                                 caAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCccagcg         >  1:447659/1‑81 (MQ=255)                                                                 caAAACGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCccagcg         <  1:447618/81‑1 (MQ=255)                                                                    aaCGTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCccagcgcca      <  2:447619/81‑1 (MQ=255)                                                                       gTGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCccagcgccagcg   >  2:447654/1‑81 (MQ=255)                                                                        tGGGCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCCCAGCGCCAGCGt  >  2:447650/1‑81 (MQ=255)                                                                           |                                                                              GACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGGAGGTATTGCGCACAAAACGTGGCCACAACTCACTACCTGGGATCAGTTCACGCAGATTAGACATCATCTGGCAACCGTTACATACCCCCAGCGCCAGCGT  >  NC_000913/2692008‑2692159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑