Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,716,500	A→G	intergenic (‑51/‑254)	grcA ← / → ung	stress‑induced alternate pyruvate formate‑lyase subunit/uracil‑DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,716,500	0	A	G	100.0%	35.7 / NA	11	intergenic (‑51/‑254)	grcA/ung	stress‑induced alternate pyruvate formate‑lyase subunit/uracil‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/7);  total (4/7)

CTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAA  >  NC_000913/2716422‑2716575                                                                               |                                                                            cTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAGtt                                                                           <  1:452304/81‑1 (MQ=255)      gTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAGTTGGTa                                                                       <  1:452305/80‑1 (MQ=255)              gATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAGTTGGTAAAACCAttg                                                              >  1:452328/1‑81 (MQ=255)              gATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAGTTGGTAAAACCAt                                                                <  2:452306/79‑1 (MQ=255)                           aTGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAGTTGGTAAAACCATTGTTGCTTGAgtgt                                                  <  1:452307/80‑1 (MQ=255)                                       cccGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAGTTGGTAAAACCATTGTTGCTTGAGTGTATATATActcc                                       <  2:452308/79‑1 (MQ=255)                                              aaaTTATTTGATTTGTTCAGCCTGTCGCGGCCAGTTGGTAAAACCATTGCTGCTTGAGTGTATATATACTCCTCAAACAcc                              >  2:452333/1‑81 (MQ=255)                                                 ttattTGATTTGTTCAGCCTGTCGCGGCCAGTTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCtt                           <  1:452309/81‑1 (MQ=255)                                                                     gTCGCGGCCAGTTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCaa       >  2:452338/1‑81 (MQ=255)                                                                      tCGCGGCCAGTTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCaa       >  2:452334/1‑80 (MQ=255)                                                                          ggCCAGTTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATaaaa  <  1:452310/81‑1 (MQ=255)                                                                               |                                                                            CTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAA  >  NC_000913/2716422‑2716575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑