Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	268,409	C→T	E184E (GAG→GAA)	ykfA ←	CP4‑6 prophage; putative GTP‑binding protein YkfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	268,409	0	C	T	100.0%	137.6 / NA	36	E184E (GAG→GAA)	ykfA	CP4‑6 prophage; putative GTP‑binding protein YkfA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

ATCCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGCTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGT  >  NC_000913/268332‑268484                                                                              |                                                                            atcCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCa                                                                          <  2:56290/81‑1 (MQ=255)   ccGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATg                                                                        >  1:56339/1‑81 (MQ=255)     gCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATgca                                                                      <  2:56291/81‑1 (MQ=255)          gCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAgcagg                                                                 <  1:56292/81‑1 (MQ=255)           ccATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAgcaggc                                                                <  2:56293/81‑1 (MQ=255)           ccATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAgcaggc                                                                <  1:56294/81‑1 (MQ=255)                aCCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGAt                                                            <  2:56295/80‑1 (MQ=255)                      aGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCgg                                                     <  2:56296/81‑1 (MQ=255)                       gCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGc                                                    >  1:136852/1‑81 (MQ=255)                         ccccATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCt                                                   >  2:56348/1‑80 (MQ=255)                               tGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttc                                            >  1:56355/1‑81 (MQ=255)                                gTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttct                                           >  1:56343/1‑81 (MQ=255)                                  aGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttctgc                                         >  2:56347/1‑81 (MQ=255)                                        aCCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttctgcttctgc                                   >  1:56342/1‑81 (MQ=255)                                        aCCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttctgcttctgc                                   <  1:56297/81‑1 (MQ=255)                                           gACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGcc                                >  2:56366/1‑81 (MQ=255)                                           gACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGcc                                <  2:56298/81‑1 (MQ=255)                                             cacaGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGt                              <  2:56299/81‑1 (MQ=255)                                              acaGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTg                             >  1:56340/1‑81 (MQ=255)                                              acaGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTg                             <  2:56300/81‑1 (MQ=255)                                              acaGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTg                             >  1:56365/1‑81 (MQ=255)                                               caGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTgg                            <  1:56301/81‑1 (MQ=255)                                                  cacacaCCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGAc                          >  2:56346/1‑80 (MQ=255)                                                     acacCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACt                      >  1:56362/1‑81 (MQ=255)                                                     acacCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACt                      >  2:56350/1‑81 (MQ=255)                                                           gATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTcccc                >  1:56344/1‑81 (MQ=255)                                                            aTGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTccccc               <  2:56302/81‑1 (MQ=255)                                                            aTGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTccccc               >  2:56360/1‑81 (MQ=255)                                                             tGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCa              >  2:56354/1‑81 (MQ=255)                                                               gACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCAcc            >  1:56361/1‑81 (MQ=255)                                                                 cGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGc          <  1:56303/81‑1 (MQ=255)                                                                  gggCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCt         <  2:56304/81‑1 (MQ=255)                                                                   ggCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTg        >  2:56363/1‑81 (MQ=255)                                                                        gAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAg    <  1:56305/80‑1 (MQ=255)                                                                        gAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAg    <  2:56306/80‑1 (MQ=255)                                                                         aaCAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGt  <  2:56307/81‑1 (MQ=255)                                                                              |                                                                            ATCCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGCTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGT  >  NC_000913/268332‑268484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑