Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,817	C→T	A238T (GCG→ACG)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,817	0	C	T	100.0%	150.2 / NA	39	A238T (GCG→ACG)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/18);  total (21/18)

CATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGC  >  NC_000913/2717739‑2717891                                                                               |                                                                           catcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtcc                                                                          >  2:452573/1‑81 (MQ=255)     cAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGa                                                                      <  2:452526/81‑1 (MQ=255)      aGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGAc                                                                     <  1:452527/81‑1 (MQ=255)         aCGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTcc                                                                  <  1:452528/81‑1 (MQ=255)         aCGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTc                                                                   >  1:452594/1‑80 (MQ=255)            ttAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcagc                                                               <  2:452529/81‑1 (MQ=255)              aaCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcagcag                                                             >  1:452564/1‑81 (MQ=255)                cAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTg                                                           >  2:452591/1‑81 (MQ=255)                 aaTGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGc                                                          >  1:452580/1‑81 (MQ=255)                  aTGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGcc                                                         <  1:452530/81‑1 (MQ=255)                   tgttgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCg                                                        >  1:452589/1‑81 (MQ=255)                      tgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCAt                                                     >  2:452561/1‑81 (MQ=255)                        tCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATcc                                                   <  1:789843/81‑1 (MQ=255)                          gCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCt                                                  <  1:452531/80‑1 (MQ=255)                          gCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCt                                                  <  1:452533/80‑1 (MQ=255)                          gCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTg                                                 <  2:452532/81‑1 (MQ=255)                           ccAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTg                                                 >  1:452581/1‑80 (MQ=255)                            cAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGca                                               >  2:452614/1‑81 (MQ=255)                                aaTCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcaccac                                           >  2:452578/1‑81 (MQ=255)                                 aTCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcaccac                                           >  1:452587/1‑80 (MQ=255)                                 aTCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcaccac                                           >  1:452535/1‑80 (MQ=255)                                 aTCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACg                                          <  2:452534/81‑1 (MQ=255)                                    ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGaca                                       >  1:452579/1‑81 (MQ=255)                                     gCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGacac                                      >  1:452577/1‑81 (MQ=255)                                         aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCt                                    <  1:452536/79‑1 (MQ=255)                                            gTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCAc                               >  1:452583/1‑81 (MQ=255)                                             tGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACg                              >  1:452595/1‑81 (MQ=255)                                                        gccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgc                   <  2:452537/81‑1 (MQ=255)                                                        gccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgc                   >  1:452586/1‑81 (MQ=255)                                                         ccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                  <  2:452538/81‑1 (MQ=255)                                                                cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCt             <  1:452540/79‑1 (MQ=255)                                                                cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCt             <  1:452541/79‑1 (MQ=255)                                                                cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgc           >  2:452584/1‑81 (MQ=255)                                                                cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTg            <  1:452539/80‑1 (MQ=255)                                                                  gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg          >  1:452592/1‑80 (MQ=255)                                                                   tACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGca        <  2:452542/81‑1 (MQ=255)                                                                     cGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcat       <  1:452543/80‑1 (MQ=255)                                                                      ggATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatca     >  2:452611/1‑81 (MQ=255)                                                                          aGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGc  >  1:452582/1‑80 (MQ=255)                                                                          aGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGc  >  2:452596/1‑80 (MQ=255)                                                                               |                                                                           CATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGC  >  NC_000913/2717739‑2717891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑