| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,810,778 | T→C | S3S (AGT→AGC) | mprA → | DNA‑binding transcriptional repressor MprA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,810,778 | 0 | T | C | 76.2% | 35.0 / 10.1 | 21 | S3S (AGT→AGC) | mprA | DNA‑binding transcriptional repressor MprA |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base C (4/12); total (5/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
AAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAG > NC_000913/2810701‑2810844 | aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGCTc < 2:469308/80‑1 (MQ=255) cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGCTCGttt < 1:469309/81‑1 (MQ=255) cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGCTCGttt < 2:469310/81‑1 (MQ=255) aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGccc < 2:469311/81‑1 (MQ=255) tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAt < 1:469312/80‑1 (MQ=255) tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAAc > 2:469344/1‑80 (MQ=255) aCTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTa < 2:469313/80‑1 (MQ=255) tCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTaaaa < 2:469314/79‑1 (MQ=255) gTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTc < 2:469315/81‑1 (MQ=255) tACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc > 2:469347/1‑81 (MQ=255) gCTGAAATTAATGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc < 1:469316/81‑1 (MQ=255) gAAATTAATGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCAc > 2:469343/1‑80 (MQ=255) aaaTTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCAc < 2:469317/79‑1 (MQ=255) ttAATGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa < 1:469318/81‑1 (MQ=255) tGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt < 1:469319/81‑1 (MQ=255) tGAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt > 2:469345/1‑81 (MQ=255) ggTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt > 2:469346/1‑81 (MQ=255) gTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt < 1:469320/81‑1 (MQ=255) aTACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt < 1:469321/80‑1 (MQ=255) tACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa < 1:469322/81‑1 (MQ=255) cccAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg < 2:469323/80‑1 (MQ=255) | AAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAG > NC_000913/2810701‑2810844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |