Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,889,110	T→C	K330K (AAA→AAG)	cysI ←	sulfite reductase, hemoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,889,110	0	T	C	100.0%	154.9 / NA	43	K330K (AAA→AAG)	cysI	sulfite reductase, hemoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/17);  total (26/17)

CAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTT  >  NC_000913/2889033‑2889184                                                                              |                                                                           cAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTg                                                                         <  1:485298/81‑1 (MQ=255)  aGTTATCATCAATGCCCTTAACCCCGCCCCTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGa                                                                        >  1:485339/1‑81 (MQ=255)    ttATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATc                                                                      >  2:485344/1‑81 (MQ=255)     tATCATCAATGCCCTTAACCCAGCCAATAAGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATcc                                                                     >  2:485373/1‑81 (MQ=255)        catcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcg                                                                  >  2:485355/1‑81 (MQ=255)        catcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcg                                                                  >  2:485360/1‑81 (MQ=255)         atcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgc                                                                 <  1:485300/81‑1 (MQ=255)         atcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcg                                                                  <  1:485299/80‑1 (MQ=255)          tcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcg                                                                >  1:485356/1‑81 (MQ=255)            aaTGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcgcg                                                              <  1:485301/81‑1 (MQ=255)              tGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGAc                                                            <  1:485302/81‑1 (MQ=255)               gCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACg                                                           >  2:485347/1‑81 (MQ=255)                     aaCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCa                                                      <  2:485303/80‑1 (MQ=255)                     aaCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCAc                                                     >  2:485367/1‑81 (MQ=255)                        ccAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTc                                                  >  2:485354/1‑81 (MQ=255)                         cAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTcc                                                 <  1:485304/81‑1 (MQ=255)                            ccAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCt                                              >  2:485353/1‑81 (MQ=255)                                     tCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGtc                                     >  2:485364/1‑81 (MQ=255)                                     tCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGtc                                     >  2:485365/1‑81 (MQ=255)                                     tCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGtc                                     <  1:485305/81‑1 (MQ=255)                                           cGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAcc                               >  2:485375/1‑81 (MQ=255)                                            gTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAccc                              <  2:485306/81‑1 (MQ=255)                                            gTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAccc                              >  2:485362/1‑81 (MQ=255)                                             tCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAcccc                             <  1:485307/81‑1 (MQ=255)                                               cGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCa                           >  2:485346/1‑81 (MQ=255)                                                 gTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAcg                         >  1:485359/1‑81 (MQ=255)                                                   gAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAcgcg                       >  1:485371/1‑81 (MQ=255)                                                   gAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAcgcg                       >  2:485374/1‑81 (MQ=255)                                                     aCTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCt                     >  2:485348/1‑81 (MQ=255)                                                     aCTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCt                     <  1:485308/81‑1 (MQ=255)                                                        cATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTc                    >  1:485366/1‑79 (MQ=255)                                                         atatGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTcc                   <  1:485310/79‑1 (MQ=255)                                                         atatGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCa                  <  1:485309/80‑1 (MQ=255)                                                         atatGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAg                 >  1:485345/1‑81 (MQ=255)                                                          tatGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGc                >  2:485349/1‑81 (MQ=255)                                                            tGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCgt              <  1:485312/81‑1 (MQ=255)                                                            tGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCgt              <  2:485311/81‑1 (MQ=255)                                                               aCGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTa           >  1:485372/1‑81 (MQ=255)                                                                 ggATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAtt         >  2:485351/1‑81 (MQ=255)                                                                 ggATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAtt         >  1:485358/1‑81 (MQ=255)                                                                 ggATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAtt         <  1:485313/81‑1 (MQ=255)                                                                    tCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTgg      <  1:485314/81‑1 (MQ=255)                                                                        ttCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGtttt  >  2:485385/1‑81 (MQ=255)                                                                              |                                                                           CAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTT  >  NC_000913/2889033‑2889184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑