Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,282	A→G	A220A (GCT→GCC)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,282	0	A	G	100.0%	120.5 / NA	34	A220A (GCT→GCC)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/17);  total (17/17)

GTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACT  >  NC_000913/2907204‑2907357                                                                               |                                                                            gTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGc                                                                           <  2:488584/81‑1 (MQ=255)    cATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGt                                                                         <  1:488585/80‑1 (MQ=255)     aTCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTg                                                                       >  2:488627/1‑81 (MQ=255)      tCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTg                                                                       >  2:488645/1‑80 (MQ=255)          cAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAAcc                                                                  >  1:488642/1‑81 (MQ=255)           aaaGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAAcc                                                                  >  2:488633/1‑80 (MQ=255)           aaaGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAAcc                                                                  >  1:488631/1‑80 (MQ=255)                 aTGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTc                                                           >  2:488639/1‑81 (MQ=255)                   gTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCgg                                                         >  2:488647/1‑81 (MQ=255)                   gTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCgg                                                         >  2:488632/1‑81 (MQ=255)                    tCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGgc                                                        <  2:488586/81‑1 (MQ=255)                       ttGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCa                                                     >  2:488625/1‑81 (MQ=255)                         gCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCAt                                                    <  1:488587/80‑1 (MQ=255)                            cAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGcc                                                <  2:488588/81‑1 (MQ=255)                               ttCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCAcc                                             <  2:488589/81‑1 (MQ=255)                               ttCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCAcc                                             >  1:488654/1‑81 (MQ=255)                                 cATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCt                                            <  2:488590/80‑1 (MQ=255)                                  aTAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCtt                                           <  1:488591/80‑1 (MQ=255)                                         gcagcTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCa                                   <  1:488592/81‑1 (MQ=255)                                                   cAGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAgtgt                         >  2:488637/1‑81 (MQ=255)                                                    aGCTTCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGtt                        <  2:488593/81‑1 (MQ=255)                                                        tCAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATg                    >  1:488662/1‑81 (MQ=255)                                                         cAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCAt                     <  1:488594/79‑1 (MQ=255)                                                         cAGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGc                   >  2:488648/1‑81 (MQ=255)                                                          aGCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCTCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGcc                  >  2:488636/1‑81 (MQ=255)                                                           gCGATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCt                 <  2:488595/81‑1 (MQ=255)                                                             gATAACAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCttt               <  1:488596/81‑1 (MQ=255)                                                                aaCAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGc            >  2:488638/1‑81 (MQ=255)                                                                  cAGCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCtt          <  1:488597/81‑1 (MQ=255)                                                                    gCCAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCttt         >  1:488643/1‑80 (MQ=255)                                                                     ccAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCttt         >  1:488628/1‑79 (MQ=255)                                                                      cAGAGCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAg      <  2:488598/81‑1 (MQ=255)                                                                        gagCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGaa    <  1:488599/81‑1 (MQ=255)                                                                          gCTTCGGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACt  <  2:488600/81‑1 (MQ=255)                                                                               |                                                                            GTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACT  >  NC_000913/2907204‑2907357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑