Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,173,177	T→A	P12P (CCT→CCA)	ygiN →	putative quinol monooxygenase YgiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,173,177	0	T	A	100.0%	86.9 / NA	24	P12P (CCT→CCA)	ygiN	putative quinol monooxygenase YgiN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/10);  total (14/10)

AATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCA  >  NC_000913/3173099‑3173254                                                                               |                                                                              aaTTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAgg                                                                             >  1:543302/1‑81 (MQ=255)  aTTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGt                                                                            <  2:543267/81‑1 (MQ=255)   tttttGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGt                                                                            >  2:543294/1‑80 (MQ=255)    ttttGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTc                                                                           >  2:543316/1‑80 (MQ=255)        ggTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACa                                                                       <  2:543268/80‑1 (MQ=255)         gTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATc                                                                     <  1:543269/81‑1 (MQ=255)         gTTAACTAGAGCTCAGGCTTTAGAAGGAATTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACAt                                                                      <  1:543270/80‑1 (MQ=255)          ttAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCa                                                                    >  2:543298/1‑81 (MQ=255)                agagCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGt                                                                >  2:543297/1‑79 (MQ=255)                     tCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCgg                                                         >  2:543307/1‑81 (MQ=255)                          cTTTAGAAGGAGTTAACCATGCTTATCGTAATCGCAGTAATCCGTACGCTTCCAGGTCAACATCGCCGTCAGACGGTATTg                                                    >  2:543319/1‑81 (MQ=255)                              aGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGAt                                                 <  2:543271/80‑1 (MQ=255)                                      ttAACCTTGCCTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGc                                         >  1:543301/1‑80 (MQ=255)                                           cATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAAt                                   <  1:543272/81‑1 (MQ=255)                                             tGCTTACCGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCg                                 >  2:543305/1‑81 (MQ=255)                                                  aCCGTAATCGCACAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTcc                             <  1:543273/80‑1 (MQ=255)                                                   ccGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCa                            <  2:543274/80‑1 (MQ=255)                                                    cGTAATCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAAc                          >  1:543312/1‑81 (MQ=255)                                                         tCGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTAc                     >  1:543293/1‑81 (MQ=255)                                                          cGCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACt                    >  1:543308/1‑81 (MQ=255)                                                           gCAGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACtt                   <  2:543275/81‑2 (MQ=255)                                                             aGAAATCCGTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGa                  >  1:543309/1‑80 (MQ=255)                                                                     gTACTCGTCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAaagaagaag         >  1:543310/1‑81 (MQ=255)                                                                            tCCAGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCa  <  1:543276/81‑1 (MQ=255)                                                                               |                                                                              AATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCA  >  NC_000913/3173099‑3173254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑