| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 294,225 | T→G | N192T (AAC→ACC) | yagM ← | CP4‑6 prophage; protein YagM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 294,225 | 0 | T | G | 75.0% | 31.6 / 7.4 | 16 | N192T (AAC→ACC) | yagM | CP4‑6 prophage; protein YagM |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base G (7/5); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
ATTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATAT > NC_000913/294145‑294300 | aTTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGt > 1:62042/1‑81 (MQ=255) aTGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGGTTGAat < 2:62023/81‑1 (MQ=255) gTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGGTTGAatat > 1:62044/1‑81 (MQ=255) tGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGGTTGAATATcc > 1:62037/1‑81 (MQ=255) tGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGGTTGAATATCCTGAGACACTTTGATGc > 1:62046/1‑81 (MQ=255) cccGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGGTTGAATATCCTGAGACACTTTGATGCt < 1:62024/80‑1 (MQ=255) tCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGGTTGAATATCCTGAGACACTTTGATGCTTTACATCa < 1:62025/81‑1 (MQ=255) cccTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGa < 1:62026/81‑1 (MQ=255) ccTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGAt < 2:62027/81‑1 (MQ=255) aaGGTTGTTCTATGAATAAGAATAATGGTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATtgta > 2:62054/1‑80 (MQ=255) ggTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATtgtaaat > 2:62053/1‑81 (MQ=255) cTATGAATAAGAATAATGGTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGtaaatgtaaatg > 2:62047/1‑81 (MQ=255) tGAATAAGAATAATGGTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATgg < 1:62028/79‑1 (MQ=255) aagaataaTGGTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTc > 1:62050/1‑81 (MQ=255) gaataaTGGTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCa > 2:62051/1‑81 (MQ=255) ataatGGTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCata < 2:62029/81‑1 (MQ=255) taatGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCatat < 1:62030/81‑1 (MQ=255) | ATTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATAT > NC_000913/294145‑294300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |