Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,175	T→C	T98A (ACC→GCC)	ribB ←	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,175	0	T	C	100.0%	145.3 / NA	40	T98A (ACC→GCC)	ribB	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/24);  total (16/24)

CGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATC  >  NC_000913/3184097‑3184250                                                                               |                                                                            cGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCa                                                                           >  2:545827/1‑81 (MQ=255)  ggCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCAc                                                                          <  1:545773/81‑1 (MQ=255)    cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg                                                                        <  2:545774/81‑1 (MQ=255)    cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg                                                                        >  2:545812/1‑81 (MQ=255)    cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg                                                                        >  2:545824/1‑81 (MQ=255)    cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg                                                                        >  2:545840/1‑81 (MQ=255)     aaTCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGt                                                                       <  1:545775/81‑1 (MQ=255)      aTCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGt                                                                       >  2:545810/1‑80 (MQ=255)       tCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTaa                                                                     <  2:545776/81‑1 (MQ=255)       tCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTa                                                                      >  1:545820/1‑80 (MQ=255)        cGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTaa                                                                     >  1:545845/1‑80 (MQ=255)          cTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAAc                                                                  <  1:545777/81‑1 (MQ=255)             cgcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAAcc                                                                 <  1:545778/79‑1 (MQ=255)              gcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGt                                                              <  1:545780/81‑1 (MQ=255)              gcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGt                                                              <  2:545779/81‑1 (MQ=255)                gAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGc                                                            <  1:545781/81‑1 (MQ=255)                     gTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAg                                                       >  1:545843/1‑81 (MQ=255)                       cGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGgc                                                     >  1:545847/1‑81 (MQ=255)                           aTACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCt                                                  <  1:545783/80‑1 (MQ=255)                           aTACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCt                                                  <  2:545782/80‑1 (MQ=255)                             aCGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgt                                               >  1:545829/1‑81 (MQ=255)                              cGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgtg                                              <  2:545785/81‑1 (MQ=255)                              cGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgt                                               <  1:545784/80‑1 (MQ=255)                               ggTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgtgt                                             >  2:545832/1‑81 (MQ=255)                                   aGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTAtt                                         >  1:545831/1‑81 (MQ=255)                                     cGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTAtttt                                       <  1:545786/81‑1 (MQ=255)                                            aaCACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACcat                                <  1:545787/81‑1 (MQ=255)                                                ccGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCAtt                            <  1:545788/81‑1 (MQ=255)                                                 cGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTg                           <  1:545789/81‑1 (MQ=255)                                                   gTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGc                         <  2:545790/81‑1 (MQ=255)                                                   gTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGc                         <  1:545791/81‑1 (MQ=255)                                                      gTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAg                       <  1:545792/80‑1 (MQ=255)                                                             cTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAgtt               <  2:545793/81‑1 (MQ=255)                                                              ttCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAgttg              >  2:545835/1‑81 (MQ=255)                                                                 aGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAgttgtt            <  2:545794/80‑1 (MQ=255)                                                                 aGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGttt           <  2:545795/81‑1 (MQ=255)                                                                   ctgctTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTAc         <  2:545796/81‑1 (MQ=255)                                                                      ctTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGc      >  2:545842/1‑81 (MQ=255)                                                                          aaTGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATc  >  2:545836/1‑81 (MQ=255)                                                                          aaTGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATc  >  1:545833/1‑81 (MQ=255)                                                                               |                                                                            CGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATC  >  NC_000913/3184097‑3184250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑