Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,216,290	G→C	P68A (CCA→GCA)	nfeF ←	NADPH‑dependent ferric chelate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,216,290	0	G	C	100.0%	118.1 / NA	33	P68A (CCA→GCA)	nfeF	NADPH‑dependent ferric chelate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/19);  total (14/19)

ATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTC  >  NC_000913/3216216‑3216365                                                                           |                                                                            aTACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGcac                                                                       <  2:552721/81‑1 (MQ=255) aTACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGca                                                                        >  1:552761/1‑80 (MQ=255)   aCAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGcaca                                                                      >  1:552799/1‑80 (MQ=255)        ggcgTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGt                                                                  >  2:552789/1‑79 (MQ=255)          cgTATAGTCACGCGACGGTGGGCGTGGTCCTACCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGc                                                              <  1:552722/81‑1 (MQ=255)             ataGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGt                                                            >  1:552791/1‑80 (MQ=255)             ataGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGt                                                            >  1:552792/1‑80 (MQ=255)                gTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAg                                                         <  2:552723/80‑1 (MQ=255)                 tCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGt                                                       <  1:552724/81‑1 (MQ=255)                       gACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAgg                                                  >  1:552772/1‑80 (MQ=255)                        aCGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGaa                                                <  2:552725/81‑1 (MQ=255)                          ggTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGa                                                 >  1:552778/1‑78 (MQ=255)                           gTGGGCGTGGTCCTTTCGGCCAGACGATGCTCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTTAGGTTGAGGAaag                                              >  2:552780/1‑80 (MQ=255)                               gCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAagag                                         >  2:552787/1‑81 (MQ=255)                               gCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAagag                                         <  2:552726/81‑1 (MQ=255)                                cGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGt                                        <  1:552727/81‑1 (MQ=255)                                cGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGt                                        <  1:552728/81‑1 (MQ=255)                                  tGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGttt                                      <  1:552729/81‑1 (MQ=255)                                    gTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGc                                    >  2:552776/1‑81 (MQ=255)                                       cTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgt                                 <  1:552730/81‑1 (MQ=255)                                          ccGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg                                <  2:552731/79‑1 (MQ=255)                                           cGGCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGAt                              <  1:552732/80‑1 (MQ=255)                                            ggCCAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCg                            >  1:552803/1‑81 (MQ=255)                                               cAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTc                          >  1:552784/1‑80 (MQ=255)                                               cAGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCa                         <  2:552733/81‑1 (MQ=255)                                                aGACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCaa                        <  2:552734/81‑1 (MQ=255)                                                 gACGATGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCa                         >  1:552805/1‑79 (MQ=255)                                                      tGCCCTCTTCCGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCAc                  <  1:552735/81‑1 (MQ=255)                                                               ccGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGt           <  1:552736/79‑1 (MQ=255)                                                               ccGTTACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTa          <  2:552737/80‑1 (MQ=255)                                                                  ttACCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAAc      <  1:552738/81‑1 (MQ=255)                                                                    aCCGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGGCAAAGCCACGCGATGTAAAACCg    >  1:552786/1‑81 (MQ=255)                                                                      cGTTGCCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTc  <  1:552739/81‑1 (MQ=255)                                                                           |                                                                            ATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTC  >  NC_000913/3216216‑3216365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑